삼지구엽초 추출물의 항균 및 항산화 활성에 대한 연구 = Antibacterial and Antioxidative Activities of Epimedium koreanum Nakai Extracts.

다국어 초록 (Multilingual Abstract)

In this study, the antibacterial and antioxidative activities of Epimedium koreanum Nakai were investigated for applications as cosmetic ingredients. Minimum inhibitory concentrations (MICs) of fraction-bacterium, that showed high antibacterial activity from disc diffusion assay on human skin pathogens, were tested. The ethyl acetate fraction on Staphylococcus aureus, Bacillus subtilis, Propionibacterium acnes and 50% ethanol extract on S. aureus exhibited higher antibacterial activities than methyl paraben, well known as a preservative. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of 3 fractions of E. koreanum Nakai were lower than (+)-α-tocopherol, known as a typical antioxidant. From the results of the scavenging activities of various ROS generated in Fe3+-EDTA/H2O2 systems (OSC50), 50% ethanol extract (OSC50 = 2.46 ± 0.06 μg/ml) and aglycone fraction (OSC50 = 1.45 ± 0.02 μg/ml) showed high activities similar to L-ascorbic acid (OSC50 = 1.50 ± 0.85 μg/ml), used as reference. The cellular protective effects (τ50) on photohemolysis by 1O2 generated by photosensitization reaction were tested. The cellular protective effect of 50% ethanol extract (τ50 = 37.0 ± 0.3 min) was similar to (+)-α-tocopherol (τ50 = 38.0 ± 1.8 min), used as reference. In particular, the τ50 of aglycone fraction results were 165.9 ± 7.2 min. This is a high cellular protective effect, more than 4 times that of (+)-α-tocopherol. These results indicate that E. koreanum Nakai extract, and its fractions, could be utilized as a cosmetic ingredient possessing antibacterial and antioxidative activities.

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