Cloning of Involution-induced cDNAs from Mouse Mammary Gland

다국어 초록 (Multilingual Abstract)

Involution-induced cDNAs from mouse mammary gland were cloned and characterized. After the primary differential screening of a total 40,000 pfu of cDNA library, 200 positive plaques were isolated. Positive plaques were rescreened by PCR/Southern differential nybridization. Two clones were characterized using Northern analysis and nucleotide sequencing. Partial sequences revealed that one clone coded for sulfated glycoprotein-2 (SGP-2). The other clone coded for WDNM1, a member of a family of protease inhibitors The expression of the SGP-2 gene was induced at involution days 1, 2, and 3. The SGP-2 was highly expressed in female mammary glands and testis. The expression levels of SGP-2 were low in thymus, uterus, ovary, liver, brain, and male mammary glands. The WDNM1 gene was induced at an early involution stage (6 h). The high levels of WDNM1 mRNA were maintained until involution day 4. The WDNM1 gene was expressed only in the mammary gland of female mice.