뽕잎오디쌀이 흰쥐의 혈당농도와 체지방 및 혈청지질 분획에 미치는 영향

국문 초록 (Abstract)

재성형 뽕잎오디혼합쌀 개발과 관련하여 당뇨유발쥐와 고지방식이를 급여한 흰쥐에게 뽕잎오디쌀이 혈당농도와 체지방 및 혈청 지질 농도 등에 미치는 영향을 조사하였다. 혈당실험에서 뽕잎오디쌀조성물은 곡류와 잡곡류 등 85%, 뽕잎과 오디즙 15%로 구성하였고, AIN-diet와 50:50 비율로 혼합하여 4주간 급여한 결과 뽕잎오디쌀 급여군은 당뇨대조군보다 실험종료 시 체중은 28% 증가한 반면 공복혈당치는 12.4%, 혈청 중성지방 농도는 34% 감소하였다(p＜0.05). 지질실험의 경우 4주 동안 고지방식이(50%)와 뽕잎오디쌀조성물(50%)의 혼합식이와 고지방식이(50%)와 기본식이(50%)의 혼합식이를 급여한 결과 뽕잎오디쌀 급여군은 고지방대조군에 비해 체중과 체지방(EFP)이 각각 8%씩 낮았고, 혈청 중성지방 농도는 약 19% 감소하였다(p＜0.05). 이상에서 뽕잎오디쌀식이는 혈당저하 및 중성지방을 감소시키는 것으로 나타났고, 유의성은 없었으나 다소간 체중과 체지방 감소 효과를 확인할 수 있었다.

다국어 초록 (Multilingual Abstract)

This study was performed to investigate the effects of rice added with mulberry leaves and fruit on blood glucose, body fat and serum lipid levels in Sprague-Dawley male rats for 4 weeks. Mulberry rice diet was prepared with mixture of 50% of purified AIN-diet and 50% of formulated rice consisting of soybean, barley, polished rice, black rice, uncleaned rice bud, mulberry leaves and fruit. The experimental animals were fed the mulberry rice diet (group A) and the purified AIN-diet (group B, diabetic control) for 4 weeks in rats injected with streptozotocin. In terms of observing hypolipidemic effect of mulberry rice, rats were fed either high-fat diet (13% fat) with additional lard, corn oil, cholesterol to AIN-diet (group C, control) or mulberry rice diet mixed with high fat diet (group D) for 4 weeks. Blood glucose level at the 4th week of group A had increased 5 ㎎/dL compared with that of day 0, while group B increased 51 mg/dL. However, the glucose levels of the groups A and B at the final day were 156 ㎎/dL (12.4% decrease compared with B) and 178 ㎎/dL, respectively. As for hypolipidemic effect, weight gain and body fat were 8% lower in the group D and serum triglyceride level also 19% lower in the group D compared with those of group C (p＜0.05).

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

1 장정연, "인진쑥 올리고당의 섭취가 비만쥐의 혈중 지질, 복강내 지방조직과 렙틴 수치에 미치는 영향" 한국영양학회 36 (36): 437-445, 2003

2 장미진, "뽕잎과 누에가루 혼합환의 Streptozotocin 유발 당뇨쥐에서의 혈당강하 효과" 한국식품영양과학회 33 (33): 1611-1617, 2004

3 Hong V, "Use of HPLC separation/photodiode array detection for characterization of anthocyanin" 38 : 708-715, 1990

4 Manninen V, "Triglycerides and LDL-cholesterol concentrations on coronary heart disease risk in the Helsinki Heart Study" 85 : 37-45, 1992

5 Lee JS, "Supplementation of whole persimmon leaf improves lipid profiles and suppresses body weight gain in rats fed high-fat diet" 44 : 1875-1883, 2006

6 Avram AS, "Subcutanous fat in normal and diseased status: 2. Anatomy and physiology of white and brown adipose tissue" 53 : 671-683, 2005

7 Ascaso JF, "Significance of HDL-cholesterol in cardiovascular risk prevention: recommendations of the HDL forum" 4 : 299-314, 2004

8 Park SW, "Quantitative analysis of anthocyanins among mulberry cultivars and their pharmacological screening" 38 : 722-724, 1997

9 Machii H, "Mulberry breeding, cultivation and utilization in Japan. FAO Electronic Conference Mulberry for Animal Production"

10 Schulz H, "Inhibitors of fatty acid oxidation" 40 : 1443-1449, 1987

11 Kim SY, "Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice" 30 : 123-129, 1999

12 Chen F, "Hypoglycemic activity and mechanisms of extracts from mulberry leaves (folium mori) and cortex mori radicis in streptozotocin-induced diabetic mice" 115 : 476-482, 1995

13 Inkeles S, "Hyperlipidemia and coronary atherosclerosis" 60 : 110-123, 1981

14 Oh H, "Hepatoprotective and free radical scavenging activities of prenylflavonoids, coumarin and stilbene from Morus alba" 68 : 932-934, 2002

15 Park JC, "Effects of the fractions from the leaves, fruits, stems and roots of Cudrania tricuspidata and flavonoids on lipid peroxidation" 26 : 377-384, 1995

16 Kim MH, "Effects of soy oligosaccharides on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats" 34 : 3-13, 2001

17 Doi K, "Effect of various fractions extracted from mulberry leaves on lipid metabolism in rabbit fed a cholesterol diet" 52 : 85-90, 1999

18 Cha JY, "Effect of potato polyphenolics on hyperlipidemia in rats" 29 : 274-279, 1999

19 Lee WC, "Effect of number of days after urea foliar spray on mulberry leaf components and silkworm" 35 : 86-88, 1993

20 Kim HB, "Effect of methanol extract from mulberry fruit on the lipid metabolism and liver function in cholesterol-induced hyperlipidemia rats" 43 : 104-108, 2001

21 Matsumoto N, "Effect of black tea polyphenols on plasma lipids in cholesterol-fed rats" 44 : 337-342, 1998

22 Oh UJ, "Effect of beverage containing extract from mulberry leaves on serum glucose and lipid levels in db/db mouse" 430-, 1999

23 Preston AM, "Diabetic parameters 58 weeks after injection with streptozotocin in rats fed basal diet supplemented with fiber, mineral and vitamin" 11 : 895-906, 1991

24 Yu SM, "Development of processed foods using mulberry fruit" Rural Resource Development Institute 1998

25 Niall MG, "Cholesterol metabolism in alloxan-induced diabetic rabbits" 39 : 626-636, 1990

26 Chung SH, "Blood glucose lowering effect of silkworm" 24 : 95-100, 1996

27 Shin DH, "Antioxidative substances in mulberry leaves" 16 : 27-31, 1998

28 Kim SY, "Antiinflammatory and antioxidative effects of Morus spp. fruit extract" 6 : 204-209, 1988

29 Kim SY, "Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats" 27 : 1217-1222, 1998

30 Reeves PG, "AIN-93 purified diets for laboratory rodent: final report of the American Institute Nutrition ad hoc writing committee in the reformulation of the AIN-76A rodent diets" 123 : 1939-1951, 1993

31 Kim TW, "A study on the antidiabetic effect of mulberry fruits" 38 : 100-107, 38