마늘의 경정배양에서 기내인경구 대량생산을 위한 2단계 배양의 도입|RISS 상세보기

다국어 초록 (Multilingual Abstract)

In vitro culture of shoot tip of garlic (Allium sativium L. cv. Seosan) was carried out to find medium condition of the induction of multiple shoots and bulbing for muliproduction of virus-free seed bulbs. For this work, tank culture was introduced. In shoot tip culture on MS solid medium the induction of multiple shoots and bulbing were better by adding 3% sucrose than 8%. Supplementation with 2 mg/L 2ip and 0.2 mg/L IAA in this medium was effective. Three point three shoots including 2.7 bulbs were formed from a shoot tip after cultivation for 30 days on this medium. Bulbing of garlic in liquid culture with plastic water tank of 20 L supplied air at the side of the lower part was better by adding 3% sucrose than 8% by subculture for 45 days with shoots obtained from shoot tip culture for 30 days on soid MS medium. Shoot growth was vigorous at 3% sucrose however bulb growth was more effective on the medium of 8% sucrose. Because of the effectiveness on solid medium added 3% sucrose, 2 mg/L 2ip and 0.2 mg/L IAA for initial production of multi-shoot in stem tip culture and the effectiveness in liquid culture with water tank for growth of bulbs, the method of two-step culture could be introduced for the multiple production of seed bulb of high quality. It was more desirable by supply of 0.2 mg/L BA and 0.02 mg/L NAA at tank culture time. But growth of the bulbs became poor by increasing concentration of NAA of the medium.

번역하기

참고문헌 (Reference)

1 Bos L., "Viruses and virus diseases of Allium species" 127 : 11-29, 1982

2 Conci V. C., "Virus free garlic (Allium sativum L.) plants obtained by thermotherapy and meristem tip culture" 132 : 186-192, 1991

3 Robert U., "Thermotherapy in virus elimination from garlic: influences on shoot multiplication from meristems and bulb formation in vitro" 73 : 193-202, 1998

4 Ayuso P., "The elimination of garlic viruses by thermotherapy and/or tissue culture" 9 : 835-836, 1981

5 Lee E. M., "Systematic propagation of high quality garlic (Allium sativum L.) through shoot apical meristem culture. Ⅱ. Effect of sucrose concentration and nitrogen source on in vitro formation of bulblets" 21 : 193-199, 1994

6 Ayabe M., "Regeneration of whole plants from protoplasts isolated from tissuecultured shoot primordia of garlic (Allium sativum L.)" 15 : 17-21, 1995

7 Moriconi D. N., "Rapid multiplication of garlic Allium" 51 : 145-151, 1990

8 Walkey D. G. A., "Production of virus-free garlic (Allium sativum L.) and shallot (A. ascalonicum L.) by meristem-tip culture" 62 : 211-220, 1987

9 Nagakubo T., "Micropropagation of garlic through in vitro bulblet formation" 32 : 175-183, 1993

10 Ravnikar M., "Jasmonic acid stimulates shoot and bulb formation of garlic in vitro" 12 : 73-77, 1993