만성폐쇄성폐질환 동물모델에서 SGX01의 폐손상 억제 효과 = Inhibitory Effects of SGX01 on Lung Injury of COPD Mice Model

다국어 초록 (Multilingual Abstract)

Objective: This study aimed to evaluate the inhibitory effects of SGX01 on the lung injuries of COPD mice model.
Materials and Methods: This study was carried out in two ways: in vitro and in vivo. In vitro, L929 cells were challenged with LPS, and then treated with six concentrations of SGX01 (10, 30, 50, 100, 300, and 500 μg/ml) and analyzed by ELISA. In vivo, C57BL/6 mice were challenged with LPS and cigarette smoking solution (CSS), and then treated with a vehicle only (control group), dexamethasone 3 mg/kg (dexa group), or a SGX01 200 mg/kg (SGX01 group). After sacrifice, the BALF or lung tissue was analyzed with Cytospin, FACS, ELISA, real-time PCR and H&E, and Masson’s trichrome staining.
Results: SGX01 significantly decreased NO, TNF-α, and IL-6 on L929 cells challenged with LPS. In the COPD model, SGX01 significantly inhibited the increase of neutrophils, TNF-α, IL-17A, CXCL-1, MIP2, CD8+ cells in BALF, and TNF-α, IL-1β mRNA expression in lung tissue. It also decreased the severity of the histological lung injury.
Conclusion: This study suggests the usability of SGX01 for COPD patients by controlling lung tissue injury.

참고문헌 (Reference)

1 이응석, "맥문동이 LPS로 유도된 폐손상에 미치는 영향" 한의병리학회 25 (25): 641-649, 2011

2 유철규, "만성폐쇄성폐질환의 병인과 병태생리" 대한내과학회 77 (77): 383-400, 2009

3 한종민, "만성폐쇄성폐질환 동물모델에 대한 射干湯 및 구성약물의 효과" 대한한의학방제학회 23 (23): 171-187, 2015

4 김인규, "熟地黃 추출물이 H2O2에 의해 유도된 ECV304 세포의 apoptosis에 미치는 영향" 한의병리학회 23 (23): 76-83, 2009

5 Churg A, "α-1-Antitrypsin ameliorates cigarette smoke-induced emphysema in the mouse" 168 (168): 199-207, 2003

6 Parker L, "Translational mini-review series on Toll-like receptors : networks regulated by Toll-like receptors mediate innate and adaptive immunity" 147 (147): 199-207, 2007

7 Barnes PJ, "The cytokine network in asthma and chronic obstructive pulmonary disease" 118 (118): 3546-3556, 2008

8 Traves S, "Th17 Cells in Airway Diseases" 8 (8): 416-426, 2008

9 Ministry of Gender Equality and Family, "Survey of Detecting Harmful Environment to Juveniles"

10 Pelkonen M, "Smoking : relationship to chronic bronchitis, chronic obstructive pulmonary disease and mortality" 14 (14): 105-109, 2008

11 Zhao ji(趙佶), "Shengjizonglu (聖濟總錄)(4)" Euisungdang 2858-2871, 1993

12 Di Stefano A, "Severity of airflow limitation is associated with severity of airway inflammation in smokers" 158 (158): 1277-1285, 1998

13 Deveci Y, "Serum ghrelin, IL-6 and TNF-αlevels in patients with chronic obstructive pulmonary disease" 58 (58): 162-172, 2010

14 황용일, "Recent Trends in the Prevalence of Chronic Obstructive Pulmonary Disease in Korea" 대한결핵및호흡기학회 80 (80): 226-229, 2017

15 Mizutani N, "Pulmonary emphysema induced by cigarette smoke solution and lipopolysaccharide in guinea pigs" 32 (32): 1559-1564, 2009

16 Barnes PJ, "New anti-inflammatory targets for chronic obstructive pulmonary disease" 12 (12): 543-, 2013

17 Lambrecht B, "Lung dendritic cells and host immunity to infection" 18 (18): 692-704, 2001

18 김용, "LPS로 유발된 만성폐쇄성폐질환에 대한 生脈淸肺飮의 영향" 대한한방내과학회 32 (32): 217-231, 2011

19 Barnes PJ, "Inflammatory mechanisms in patients with chronic obstructive pulmonary disease" 138 (138): 16-27, 2016

20 Rovina N, "Inflammation and immune response in COPD:where do we stand?" 2013 : 2013

21 Traves S, "Increased levels of the chemokines GROαand MCP-1 in sputum samples from patients with COPD" 57 (57): 590-595, 2002

22 Profita M, "In vitro anticholinergic drugs affect CD8+peripheral blood T-cells apoptosis in COPD" 217 (217): 345-353, 2012

23 Culpitt SV, "Impaired inhibition by dexamethasone of cytokine release by alveolar macrophages from patients with chronic obstructive pulmonary disease" 167 (167): 24-23, 2003

24 Levänen B, "Impact of tobacco smoking on cytokine signaling via interleukin-17A in the peripheral airways" 11 : 2109-, 2016

25 Lee HJ, "Herbal formula, PM014, attenuates lung inflammation in a murine model of chronic obstructive pulmonary disease" 2012 : 2012

26 Aaron SD, "Granulocyte inflammatory markers and airway infection during acute exacerbation of chronic obstructive pulmonary disease" 163 (163): 349-355, 2001

27 Gebrehiwot TT, "Global, regional, and national deaths, prevalence, disability-adjusted life years, and years lived with disability for chronic obstructive pulmonary disease and asthma 1990-2015: a systematic analysis for the Global Burden of Disease Study 2015 (vol 5, pg 691 2017)" 5 (5): E30-, 2017

28 Global Initiative for Chronic Obstructive Lung Disease, "Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Pulmonary Disease 2019 Report"

29 Nowrin K, "Epithelial-mesenchymal transition as a fundamental underlying pathogenic process in COPD airways : fibrosis, remodeling and cancer" 8 (8): 547-559, 2014

30 이주관, "Elastase로 유도된 폐손상에 대한 小靑龍湯의 보호효과" 대한한의학회 32 (32): 83-99, 2011

31 김형원, "Elastase 매개성 폐조직 손상에 대한 麥門冬湯의 보호효과" 대한한의학회 32 (32): 63-78, 2011

32 양원경, "Effects of GHX02 on Chronic Obstructive Pulmonary Disease Mouse Model" 대한한의학회 39 (39): 126-135, 2018

33 Lukacs NW, "Chemokines and their receptors in chronic pulmonary disease" 4 (4): 313-317, 2005

34 이응석, "COPD 동물 모델에서 소청룡탕 흡입제형의 효과" 대한한의학회 34 (34): 54-68, 2013

35 Herbology Editorial Committee of Korean Medicine schools, "Boncho-hak" Younglimsa 124-125, 1991

36 Ryu MS, "Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk" 115 (115): 184-193, 2008