모링가 추출물에 대한 화장품약리활성 검증 = Studies on Cosmeceutical Activity of Extracts of Moringa oleifera Extract

다국어 초록 (Multilingual Abstract)

The purpose of this study was to investigate the role of the Moringa oleifera (M. oleifera) extract as a cosmetic additive. The tyrosinase and elastase inhibitory effects showed 47% and 39% at 1,000 μg/mL concentration, respectively. Also, the collagenase inhibition effect was 31% at 500 μg/mL concentration. A cell viability test, measured on macrophage cell (RAW 264.7) and melanoma cell (B16F10) by ethanol extract of M. oleifera, showed 94.2% and 94.8% at 100 μg/mL concentration, respectively. In order to confirm anti-inflammatory activity, we examined the inhibitory effects on the production of lipopolysaccharides (LPS)-induced NO in RAW 264.7 cells by Griess assay. As a result, the M. oleifera extract showed a concentration-dependent inhibition of NO production. The protein expression inhibitory effects of M. oleifera extract were measured by western blot at 25, 50, 100 μg/mL concentration and the β-actin. Results showed that the expression inhibition rates of the iNOS, COX-2, MITF, TRP-1, TRP-2, tyrosinase protein were decreased by 85.8%, 57.5%, 80.7%, 30%, 29.9%, 23.6% at 100 μg/mL concentration, respectively. It was concluded that M. oleifera extracts had the anti-inflammatory and whitening effects and thus could be applied for cosmetics as a natural ingredient.

참고문헌 (Reference)

1 최미은, "홍삼 교맥 복합물의 미용식품 응용 가능성에 대한 연구 : 항산화 및 Collagenase 저해 효과" 대한한의학방제학회 17 (17): 153-162, 2009

2 이정미, "한방화장품 소비자의 구매행동이 브랜드태도, 쇼핑만족 및 추천의도에 미치는 영향" 한국패션비즈니스학회 15 (15): 129-144, 2011

3 기현희, "아로니아 및 모링가 종자 복합물의 항아토피 상승효과" 한국식품영양과학회 46 (46): 298-305, 2017

4 허용, "실내환경 주요 호흡기 알레르기 항원 감작검사를 위한 피부단자시 험과 혈청 ELISA시험의 상관성에 대한 연구" 한국환경보건학회 34 (34): 369-373, 2008

5 정다현, "미역(Undaria pinnatifida) 물 추출물의 염증 억제 활성" 한국응용생명화학회 55 (55): 221-225, 2012

6 최영주, "모링가 잎 추출물의 항당뇨, 알코올 대사 및 간 보호 활성" 한국식품영양과학회 45 (45): 819-827, 2016

7 E. Wunsch, "Zur quantitativen bestimmung der kollagenase" 333 (333): 149-, 1963

8 C. S. Williams, "The role of cyclooxygenases in inflammation, cancer, and development" 18 (18): 7908-, 1999

9 J. Rodriguez-Vita, "The resolution of inflammation and cancer" 21 (21): 61-, 2010

10 N. Smit, "The hunt for natural skin whitening agents" 10 (10): 5326-, 2009

11 D. O. Adams, "The cell biology of macrophage activation" 2 : 283-, 1984

12 M. Seiji, "Subcellular localization of melanin biosynthesis" 100 (100): 497-, 1963

13 H. Ukeda, "Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'--1--(phenylamino)-carbonyl--3, 4-tetrazolium]-bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase" 251 (251): 206-, 1997

14 M. Wlaschek, "Solar UV irradiation and dermal photoaging" 63 (63): 41-, 2001

15 R. J. Cannell, "Results of a large scale screen of microalgae for the production of protease inhibitors" 54 (54): 10-, 1988

16 D. J. Stuehr, "Purification and characteriazation of the cytokine-induced macrophage nitric oxide syn- thase: an FAD and FMN containing flavoprotein" 88 (88): 7773-, 1991

17 D. L. DeWitt, "Orientation of the active site and antigenic determinants of prostaglandin endoperoxide of synthase in the endoplasmic reticulum" 256 (256): 10375-, 1981

18 M. Miyataka, "Nitric oxide, anti-inflammatory drugs on renal prostaglandins and cyclooxygenase-2" 39 (39): 785-, 2002

19 B. G. Knowles, "Nitric oxide synthases in mammals" 298 (298): 249-, 1994

20 R. M. Palmer, "Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor" 327 (327): 524-, 1987

21 R. Korhonen, "Nitric oxide production and signaling in inflammation" 4 (4): 471-, 2005

22 F. Anwar, "Moringa oleifera: a food plant with multiple medicinal uses" 21 (21): 17-, 2007

23 F. S. Laroux, "Mechanisms of inflammation: the good, the bad and the ugly" 9 : 3156-, 2004

24 M. G. Ryo, "KDA textbook editiong board, Dermatology, 5" 348-, 2008

25 M. El-Domyati, "Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin" 11 (11): 398-, 2002

26 A. Yagi, "Inhibition of mushroom-tyrosinase by aloe extract" 53 (53): 515-, 1986

27 J. K. Kundu, "Inflammation: gearing the journey to cancer" 659 (659): 15-, 2008

28 C. Nathan, "Inducible nitric oxide synthase: what difference does it make?" 100 (100): 2417-, 1997

29 K. Maeda, "In vitro effectiveness of several whitening cosmetic components in human melanocytes" 42 : 361-, 1991

30 S. P. Jeroma, "Identification of collagen fibrils in scleroderma skin" 90 (90): 48-, 1998

31 G. E. Costin, "Human skin pigmentation: melanocytes modulate skin color in response to stress" 21 (21): 976-, 2007

32 이효진, "HaCaT 각질형성세포에서 TNF-α에 의하여 유도되는 염증 발현에 대한 부위별 모링가 추출물의 억제 효과" 한국생명과학회 22 (22): 1254-1260, 2012

33 P. U. Giacomoni, "Factors of skin ageing share common mechanisms" 2 (2): 219-, 2001

34 J. Carmichael, "Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing" 47 (47): 936-, 1987

35 E. Y. Kwak, "Effect of TPA on MIFT (microphthalmia-associated transcription factor) protein chip and melanogenesis in B16 melanoma cell" 2006

36 M. E. Turini, "Cyclooxygenase-2: a therapeutic target" 53 : 35-, 2002

37 D. O. Lim, "Cosmetics industry analysis report, 57" Korea Health Industry Development Institute 2011

38 L. C. Green, "Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids" 126 (126): 131-, 1982

39 A. A. Hamza, "Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats" 48 (48): 345-, 2009

40 S. Im, "Activation of the cyclic AMP pathway by ${\alpha}$-melanotropin mediates the rest of human melanocytes to ultraviolet B radiation" 58 (58): 47-, 1998

41 R. Paliwal, "A review on horse radish tree (Moringa oleifera): a multipurpose tree with high economic and commercial importance" 3 (3): 317-, 2011

42 T. Kushimoto, "A model for melanosome biogenesis based on the purification and analysis of early melanosomes" 98 (98): 10698-, 2001