MC3T3-E1세포주에서 BHH10의 골 형성 효능 연구

다국어 초록 (Multilingual Abstract)

Objectives: BHH10 is traditional medicine herb used for enhancing body resistance against various diseases. The aim of this study was to identify BHH10 extract induces osteogenic activity in human osteoblast-like MC3T3-E1 cells.
Methods: MC3T3-E1, pre-osteoblast cell line, were treated with BHH10 of various concentrations (0.1㎍/mL, 1㎍/mL, 10㎍/mL). And then, the effect of BHH10 on osteoblast differentiation was examined by alkaline phosphatase (ALP) activity, von Kossa staining and RT-PCR for osteoblast differentiation markers such as osteocalcin (OCN) , osteopontin (OPN).
Results: BHH10 had dose-dependent effect on the viability of osteoblastic cells, and dose-dependently increased alkaline phosphatase (ALP) activity. BHH10 markedly increased mRNA expression for OCN, OPN in MC3T3-E1 cells. Also, BHH10 significantly induced mineralization in the culture of MC3T3-E1 cells.
Conclusions: In conclusion, these results propose that BHH10 can play an important role in osteoblastic bone formation, osteogenesis, and may possibly lead to the development of bone-forming drugs.

목차 (Table of Contents)

• I. 서론 1
• II. 방법 3
• 2.1 혼합생약(BHH10) 추출물의 제조 3
• 2.2. 계대 배양 3
• 2.3. 조골세포주의 시간별 분화 3
• 2.3.1 Alkaline phosphatase staining 3
• 2.3.2 von Kossa staining 4
• 2.3.3 RT-PCR analysis 4
• 2.4. 조골세포주에 BHH10 약제처리 6
• 2.4.1 Cell proliferation 6
• 2.4.2 Alkaline phosphatase staining 6
• 2.4.3 von Kossa staining 6
• 2.4.4 RT-PCR analysis 6
• 2.5. 통계 처리 7
• III. 결과 8
• 3.1. 조골세포주 분화에 따른 시간별 ALP변화 8
• 3.2. 조골세포주 분화에 따른 시간별 mineralization 8
• 3.3. 조골세포주 분화에 따른 시간별 골 형성 관련 유전자 발현 9
• 3.4. 조골세포주에서의 BHH10 세포생존률 10
• 3.5. BHH10처리된 조골세포주의 ALP 11
• 3.6. BHH10처리된 조골세포주의 mineralization w13
• 3.7. BHH10의 조골세포주 골 형성 관련 유전자 14
• IV. 고찰 16
• V. 결론 19
• Ⅵ. 참고문헌 20
• ABSTRACT 24