비만세포에서 용초와 양제근 복합처방의 알레르기 염증 억제 효과

다국어 초록 (Multilingual Abstract)

The prevalence of allergic inflammation conditions such as allergic asthma, atopic dermatitis, and allergic rhinitis is increasing among modern-day people. These chronic diseases are common for both men and women of all ages, causing discomfort in everyday lives. In previous study, Rumecis Radix and Cordyceps militaris formula (YYF) have an anti-inflammatory effect on activated human mast cells. However, a regulatory effect of YYF on mast cell proliferation induced by thymic stromal lymphopoietin (TSLP) remains unclear. To identify an anti-proliferative effect and the fundamental mechanism of YYF, the author used a human mast cell line HMC-1. YYF increased the release of p53 and Bax. It also restored caspase-3 activity reduced by TSLP. YYF suppressed the release of murine double minute-2 protein, which is related to the regulation of p53 protein. Also, it decreased the level of interleukin (IL)-13 protein and mRNA. These findings suggest that YYF can be used as an effective material when developing an anti-inflammatory agents.

목차 (Table of Contents)

• Ⅰ. 緖 論 1
• Ⅱ. 材料 및 方法 3
• 1. YYF의 준비 3
• 2. 세포배양 3
• 3. Bromodeoxyuridine (BrdU) 분석 4
• 4. MTT assay 4
• 5. Western blot 분석 5
• 6. Caspase-3 assay 5
• 7. Enzyme-linked immunosorbent assay (ELISA) 분석 6
• 8. RNA 분리 및 RT-PCR 분석 6
• 9. 통계학적 분석 7
• Ⅲ. 結 果 8
• 1. 비만세포 증식에 있어 YYF의 효과 8
• 2. 세포사멸(apoptosis)관련 단백질 발현에 있어 YYF의 효과 10
• 3. Caspase-3의 활성에 있어 YYF의 효과 11
• 4. MDM2 발현에 있어 YYF의 효과 13
• 5. IL-13 발현에 있어 YYF의 효과 15
• Ⅳ. 考察 16
• Ⅴ. 結論 19
• 參考文獻 20
• ABSTRACT 25