뼈흡수유도호르몬이 ROS17/2.8세포로부터 Nitric Oxide 형성에 미치는 영향 = Effects of osteotropic hormones on the nitric oxide production in culture of ROS17/2.8 cells

다국어 초록 (Multilingual Abstract)

Purpose : We performed the present study to investigate whether osteotropic hormomes play roles on the nitric oxide (NO) production in culture of ROS17/2.8 osteoblastic cells.
Materials and Methods : The osteoblastic cell line ROS17/2.8 cells were cultured in F12 medium supplemented with 5% fetal bovine serum (FBS) at 37?C in a humidified atmosphere of 5% CO₂ in air. ROS17/2.8 cells were plated in 96-well plates at a density of 2-3×10³ cells/well and grown to confluence. Then the cells were pretreated with osteotropic hormones (parathyroid hormone (PTH) 20-500 ng/mL, 1,25 dihydroxycholecalciferol (1,25[OH]₂D₃) 1-100 nM; prostaglandin E₂ (PGE₂) 20-500 ng/mL) in the medium supplemented with 0.4% FBS for 72 hours and the cells were treated with cytokines (TNFα and IFNγ) in phenol red-free F12 medium for an additional 48 hours. NO synthesis was assessed by measuring the nitrite anion concentration, the reaction product of NO, in the cell culture medium using Griess reagent.
Results : PTH and 1, 25[OH]₂D₃ pretreatment induced a significant increase in NO production in the presence of TNFα and IFNγ. PGE₂ slightly induced NO production compared to the control group. But, PGE2 pretreatment did not affect in NO production in the presence of TNFα and IFNγ.
Conclusions : These results suggest that the actions of osteotropic hormones in bone metabolism may be partiallymediated by NO in the presence of cytokines.

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