6-Dimethylaminopurine 및 Cycloheximide의 처리가 돼지난포란의 단위발생에 미치는 영향 = Effect of 6-Dimethylaminopurine and Cycloheximide Treatments on Parthenogenetic Development of Porcine Follicular Oocytes

다국어 초록 (Multilingual Abstract)

This study is aimed at finding an optimum density for 6-Dimethylaminopurine (6-DMAP) and cycloheximide which have an effect on the revitalization of porcine oocytes. The results were summarized as follows:
1. When 6-DMAP was treated with 2 mM for 2 hours. It showed a significantly (P<0.05) different high result in activation rate, cleavage rate and blastocyst growth rate of 51.2%, 52.7% and 25.2% respectively.
2. When Cycloheximide was treated with 5 ug/ml for 6 hours, It showed a significantly (P <0.05) different high result in activation rate, cleavage rate and blastocyst growth rate of 47.7%, 46.8%, and 27.3% respectively.
3. When it was cultured in the culture medium, NCSU, for 7 days after inducing activation with 6-DMAP and cycloheximide, it showed no differences in the number of inner cell mass (ICM) and total cell of blastocysts.
To conclude, it has been examined for porcine oocytes to be suitable when 6-DMAP was treated with 2 mM for 2 hours, Cycloheximide with 5 ug/ml for 6 hours.