Objectives and methods: Study the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Evodiae Fructus infusion solution, we injected Evodiae Fructus infusion solution into Zusanli(St36) of C57BL/6 mouse whic...
Objectives and methods: Study the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Evodiae Fructus infusion solution, we injected Evodiae Fructus infusion solution into Zusanli(St36) of C57BL/6 mouse which is corresponding to human Zusanli(St36). We observed its effect on the number of CD25+/CD4+, CD8+/CD3e+, CD69+/B220+, NK1.1+/CD3e+ cells in mouse PBMCs, the number of the pulmonary colony, MST and ILS of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results and Conclusions: The spleen cells proliferation of the sample groups treated with EDR-HAS extract has increased significantly compared with that of the control group. The percentage of the CD25+/CD4+, CD8+/CD3e+, CD69+/B220+, NK1.1+/CD3e+ cells in C57BL/6 mouse PBMCs of the sample groups treated with EDR herbal-acupuncture has increased compared with that of the control group. The lung colony number of the sample groups treated with EDR herbal-acupuncture has decreased significantly compared with that of the control group. MST and ILS of the sample groups treated with EDR herbal-acupuncture have increased significantly compared with those of the control group.