Identification of Genes Induced in Wound-treated Wild Rice (Oryza minuta)

다국어 초록 (Multilingual Abstract)

A subtracted library was constructed from wound-treated wild rice (Oryza minuta) by suppression subtrac-tive hybridization (SSH) in combination with mirror orientation selection (MOS). To distinguish between differentially expressed transcripts and false positive clones, DNA chips containing 960 random clones were applied as a form of reverse Northern screening. Based on the signal intensities and expression ratios obtained from experiments performed in triplicate, 371 clones were selected. ESTs produced from the subtracted li-brary showed 63.2% redundancy, and 72% of all clones could be matched to the GenBank nonredundant data-base. Functional categorization placed the identified enriched genes in categories of subcellular localization, metabolism, cell rescue and defense, and transcription. These EST-related resistance mechanisms could be used in investigations into the defense mechanisms of wild species, and to provide new routes to improving the germplasm of cultivated rice.

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