Optimization of the microemulsion formulation of curcuma oil and evaluation of its acaricidal efficacy against Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae)

다국어 초록 (Multilingual Abstract)

The microemulsion formulation (hereafter formulation) of curcuma oil and its acaricidal efficacy against Tetranychus cinnabarinus Boisduval (Acari: Tetranychidae) were optimized in the laboratory to evaluate their spray effectiveness of oviposition inhibition and repellence. Ethovision XT6 was used to analyse the effects of the sublethal concentrations (LC 20 ) of curcuma oil and the formulation on the behaviors of T. cinnabarinus. The results showed that Tween-80 was the best surfactant, Isopropanol was the best co-surfactant and K m = 2:1 was the best condition for the formulation. The prepared microemulsions are stable under conditions of centrifugation and incubation for extended periods. The results showed that the effect of the spray bioassays of the formulation against T. cinnabarinus continuously increased during the experiment, but for curcuma oil almost no longer increase observed when the exposure time went beyond 24 h. Moreover, compared with curcuma oil (LC 50 = 0.716%), the spray bioassay of the formulation (LC 50 = 0.035%) was stronger against T. cinnabarinus.
The repellency of the formulation to T. cinnabarinus was stronger with increasing exposure time, but that of curcuma oil declined after 12 h of exposure. The mobile distance of T. cinnabarinus treated with the formulation continuously declined during the experiment but that due to the curcuma oil almost no longer declined when the treatment time reached 12 h. The maximum mobile frequency of T. cinnabarinus treated by curcuma oil and the formulation was recorded at 6 h and 12 h, respectively. Thus, the formulation is a promising candidate as a botanical acaricide of green vegetables.

참고문헌 (Reference)

1 Eddins, D., "Zebrafish provide a sensitive model of persisting neurobehavioral effects of developmental chlorpyrifos exposure : comparison with nicotine and pilocarpine effects and relationship to dopamine deficits" 32 : 99-108, 2010

2 Aslan, İ., "Toxicity of essential oil vapours to two greenhouse pests, Tetranychus urticae Koch and Bemisia tabaci Genn" 19 : 167-173, 2004

3 Desneux, N., "The sublethal effects of pesticides on beneficial arthropods" 52 : 81-106, 2007

4 Ahmad, S. N. B., "Study of tannery phase diagram of rotenone Derris elliptica(Fabales : Fabaceae) : a novel approach of insecticide formulation" 25 : 4718-4722, 2013

5 Liu, H. X., "Studies on the chemical components of volatile oil extracting from Curcuma Longa and Turmeric Oleoresin" 40 : 247-260, 2007

6 Tunc, I., "Sensitivity of two greenhouse pests to vapours of essential oils" 86 : 183-187, 1998

7 Al-Adham, I. S. I., "Microemulsions are membrane-active, antimicrobial, self-preserving systems" 89 : 32-39, 2000

8 He, S. K., "Microemulsification of clove essential oil improves its in vitro and in vivo control of Penicillium digitatum" 65 : 106-111, 2016

9 Ostertag, F., "Low-energy formation of edible nanoemulsions : Factors influencing droplet size produced by emulsion phase inversion" 388 (388): 95-102, 2012

10 Almeida, J. R., "Linking behavioural alterations with biomarkers responses in the European seabass Dicentrarchus labrax L. exposed to the organophosphate pesticide fenitrothion" 19 : 1369-1381, 2010

11 Rao, J., "Lemon oil solubilization in mixed surfactant solutions : rationalizing microemulsion and nanoemulsion formation" 26 : 268-276, 2012

12 Pant, M., "Insecticidal activity of eucalyptus oil nanoemulsion with karanja and jatropha aqueous filtrates" 91 : 119-127, 2014

13 Duarte, J. L., "Evaluation of larvicidal activity of a nanoemulsion of Rosmarinus officinalis essential oil" 25 (25): 189-192, 2015

14 Chrysargyris, A., "Effects of water stress on lavender and sage biomass production, essential oil composition and biocidal properties against Tetranychus urticae(Koch)" 213 : 96-103, 2016

15 Cheng, Z. H., "Effects of patchouli and wormwood oils on the bioassays and behaviors of Tetranychus cinnabarinus(Boisduval)(Acari : Tetranychidae)" 66 : 271-278, 2020

16 Laborda, R., "Effects of Rosmarinus officinalis and Salvia officinalis essential oils on Tetranychus urticae Koch(Acari : Tetranychidae)" 48 : 106-110, 2013

17 Landeros, M. N., "Effect of sublethal concentrations of avermectin on population parameters of Tetranychus urticae on strawberry" 27 (27): 283-289, 2002

18 Cavalcanti, S. C. H., "Composition and acaricidal activity of Lippia sidoides essential oil against two-spotted spider mite (Tetranychus urticae Koch.)" 101 : 829-832, 2010

19 Nenaah, G. E., "Chemical composition, toxicity and growth inhibitory activities of essential oils of three Achillea species and their nano-emulsions against Tribolium castaneum(Herbst)" 53 : 252-260, 2014

20 Nenaah, G. E., "Chemical composition, insecticidal activity and persistence of three Asteraceae essential oils and their nanoemulsions against Callosobruchus maculatus (F.)" 61 : 9-16, 2015

21 Deng, K. P., "Carcass traits, meat quality, antioxidant status and antioxidant gene expression in muscle and liver of Hu lambs fed perilla seed" 102 (102): 828-837, 2018

22 Ding, L. J., "Bioguided fractionation and isolation of esculentoside P from Phytolacca americana L" 44 : 534-541, 2013

23 Pandey, A., "Antitermitic activity of plant essential oils and their major constituents against termite Odontotermes assamensis Holmgren(Isoptera : Termitidae)of North East India" 75 : 63-67, 2012

24 Severino, R., "Antibacterial and physical effects of modified chitosan based-coating containing nanoemulsion of mandarin essential oil and three non-thermal treatments against Listeria innocua in green beans" 191 : 82-88, 2014

25 Yang, Q., "Analysis on volatile oils from Curcuma longa Linn in Hainan province in different harvest period by GC-MS" 7 : 28-30, 2016

26 Xin, T., "Alterations in antioxidant enzyme activities and lipid peroxidation induced by diflubenzuron in the carmine spider mite, Tetranychus cinnabarinus, (Boisduval)(Acari : Tetranychidae)" 43 (43): 366-373, 2017