에이코사펜타인산이 돼지난포란의 체외 성숙에 미치는 영향

다국어 초록 (Multilingual Abstract)

Among fatty acid families, the polyunsaturated fatty acids were demonstrated to be mediators in various reproductive processes as precursor of steroid hormone (via cholesterol) and prostaglandins (via arachidonic acid), and in the last decade, major research was focused on the effects of omega-6 and especially omega-3 fatty acid. Eicosapentaenoic acid, the longest members of omega-3 fatty acid family, can be produced by a series of desaturation and elongation reactions from shorter member such as α-Linolenic acid. However, very few studies have provided detailed descriptions of Eicosapentaenoic acid effects and mechanisms of action in mammalian oocytes. The purpose of this study was to evaluate the effect of Eicosapentaenoic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of Eicosapentaenoic acid was added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, nuclear maturation rate, blastocysts quality, and levels of prostaglandin E2, 17β-estradiol, progesterone in the spent medium. High doses (100 mM) of Eicosapentaenoic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 mM Eicosapentaenoic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of E2/P4 also significantly increased compared with control group (p < 0.05). However, Supplementation of 100 mM Eicosapentaenoic acid showed high apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17b-estradiol/progesterone also significantly decreased compared with control group (p < 0.05). Our results indicated that supplementation with appropriate levels of Eicosapentaenoic acid beneficially affects the change of hormone synthesis for controlling oocyte maturation, leading to improved embryo quality. However, high doses of Eicosapentaenoic acid treatment results in detrimental effects.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 기초 배양액
• 돼지 난포란의 회수 및 체외 성숙(IVM: in vitro maturation)
• 체외 수정(IVF: in vitro fertilization)
• 체외 배양(IVC: in vitro culture)
• 난구세포의 팽창 정도와 핵 성숙
• 체외 성숙 배양액 내의 PGE2 농도 조사
• 체외 성숙 배양액 내의 E2와 P4 농도 조사
• 체외 생산된 배반포의 품질 조사(TUNEL assay: terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling assay)
• 통계 처리
• 결과
• 체외 성숙시 EPA 첨가가 난포란의 난구세포 팽창에 미치는 효과
• 체외 성숙시 EPA 첨가가 난포란의 핵 성숙에 미치는 효과
• 체외 성숙시 EPA 첨가가 PGE2 합성에 미치는 효과
• 체외 성숙시 EPA 첨가가 체외 수정 후 난 분할율과 배반포로의 체외 발달에 미치는 효과
• 체외 성숙시 EPA 첨가가 체외 생산된 배반포의 품질에 미치는 효과
• 체외 성숙시 EPA 첨가가 E2와 P4의 합성 및 그 비율에 미치는 효과
• 고찰
• 결론
• CONFLICTS OF INTEREST
• ACKNOWLEDGEMENTS
• ORCID
• REFERENCES