완충 용액의 유산 농도와 pH가 법랑질의 재광화에 미치는 영향 = THE EFFECT OF LACTIC ACID CONCENTRATION AND pH OF LACTIC ACID BUFFER SOLUTIONS ON ENAMEL REMINERALIZATION

다국어 초록 (Multilingual Abstract)

There are considerable in vitro and in vivo evidences for remineralization and demineralization occurring Simul-taneously in incipient enamel caries. In order to "heal" the incipient dental caries, many experiments have been carried out to determine the optimal conditions for remineralization. It was shown that remineralization is affected by different pH, lactic acid concentrations, chemical composition of the enamel, fluoride concentrations, etc.
Eighty specimens from sound permanent teeth without demineralization or cracks, 0.15 mm in thickness, were immersed in lactic acid buffered demineralization solutions for 3 days. Dental caries with a surface zone and subsurface lesion were artificially produced. Groups of 10 specimens were immersed for 10 or 12 days in lactic acid buffered remineralization solutions consisting of pH 4.3 or pH 6.0, and 100, 50, 25, or 10 mM lactic acid. After demineralization and remineralization, images were taken by polarizing microscopy (×100) and micro-computed tomography. The results were obtained by observing images of the specimens and the density of the caries lesions was determined.
1.As the lactic acid concentration of the remineralization solutions with pH 4.3 was higher, the surface zone of the carious enamel increased and an isotropic zone of the subsurface lesion was found. However, the total decalcification depth increased at the same time
2.In the remineralization solutions with pH 6.0, only the surface zone increased slightly but there was no significant change in the total decalcification depth and subsurface zone.
In the lactic acid buffer solutions with the lower pH and higher lactic acid concentration, there were dynamic changes at the deep area of the dental carious lesion.

국문 초록 (Abstract)

본 연구의 목적은 유산 완충 용액의 pH및 유산 농도 변화가 법랑질에서 인공 우식 병소의 재광화 양상에 미치는 영향을 평가하기 위함이다. 유산 완충 용액을 이용하여 법랑질을 탈회시키고, 유산의 농도가 100, 50, 25, 10 mM이고 pH가 4.3인 네 가지 재광화 용액 (Group 1, 2, 3, 4)에 10일, pH 6.0인 네 가지 재광화 용액 (Group 5, 6, 7, 8)에 12일간 처리한 후 무기질의 양적 변화를 편광 현미경에서 얻은 상에서의 탈회 깊이의 변화, 우식 표면층 깊이의 변화로 측정하였다. 또한 micro-computed tomography (micro-CT)를 이용하여 탈회 후와 재광화 후를 촬영하여 얻은 상으로부터 lesion부위의 density를 비교하였다.
1. pH 4.3에서 유산 농도가 증가할수록 우식 표면층의 증가와 표층하 탈회부 중 심층부의 isotropic zone등의 재광화 현상을 보이나, 전체 탈회 깊이 역시 증가하였다.
2. pH 6.0에서 우식 표면층에 국한된 재광화 현상을 보이고, 전체 탈회 깊이는 변화가 없었다. 이상의 실험 결과로 미루어 포화도가 일정한 유산 완충 용액으로 재광화 유도시 pH가 낮고 유산의 농도가 높을수록 표면으로부터 심층부 동역학적 변화에 더 영향을 주었으며, micro-CT를 이용한 우식 병소부위의 density 확인을 통해 무기질의 침착과 방출 현상을 객관적으로 평가할 수 있었다.

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