잔디종자의 물리적 전처리가 캘러스 형성과 식물체 재분화에 미치는 효과 = Effect of Physical Pre-treatment of Mature Seed in Callus Formation and Plant Regeneration of Zoysiagrass

다국어 초록 (Multilingual Abstract)

In order to improve the transformation efficiency of zoysiagrass by increasing the frequency of callus formation from mature seeds and plant regeneration, the effect of pre-treatment with sea sand was examined. Mature zoysiagrass seeds were given 10 min of swelling time before sea sand treatment using a sea sand and seed ratio of 1 : 1 and a vortex shaking speed of 6 (1,000 rpm) for 10 min. The seeds showed increased callus formation that was more than 2 times the rate in the control. In addition, plant regeneration efficiency was also increased when embryogenic callus induced from sea sand-treated mature seeds was cultured in regeneration medium. These results will be very helpful for improving the genetic transformation frequency of zoysiagrass, a recalcitrant monocot grass.

참고문헌 (Reference)

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