능이버섯(Sarcodon aspratus) 추출물의 항산화성과 항균성

국문 초록 (Abstract)

본 연구는 식품, 약용, 유전자원 등 풍부한 가치를 가지고 있는 능이버섯에 대하여 기능성 식품의 개발타당성을 입증하기 위하여 능이버섯을 열수와 에탄올로 추출하고 생리활성효과를 측정하였다. 과산화물가(POV)는 항산화제로 사용되는 α-tocopherol과 BHT보다 능이버섯 에탄올추출물이 높은 항산화 작용을 나타내었다. 능이버섯 추출물의 전자공여능을 측정한 결과는 능이버섯 추출물의 농도에 비례하여 전자공여능이 증가하였으며, 열수추출물이 에탄올추출물보다 전자공여능이 높았다. 능이버섯 추출물의 pH에 따른 아질산염 분해능과 농도에 따른 아질산염 분해능을 조사한 결과 추출물의 농도가 증가할수록 아질산염 분해능이 증가하였고, 열수추출물이 에탄올추출물보다 분해작용이 좀 더 높게 나타났다. 능이버섯의 SOD 유사활성은 에탄올추출물이 열수추출물보다 높게 나타내었다. 추출물에 대한 항균활성은 6종류의 식중독세균 Bacillus subtilis KCTC 1659, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 13565, Gram 음성균인 Escherichia coli O157:H7 ATCC 43895, Salmonella Typhimurium ATCC 7988, Shigella sonnei KCTC 2009을 실험균주로 사용하였다. 능이버섯의 항균활성은 열수추출물과 에탄올추출물 모두 3%, 5% 농도에서 양성균인 L. monocytogenes와 S. aureus에 대하여 항균활성을 나타내었다.

다국어 초록 (Multilingual Abstract)

The antioxidative and antimicrobial activities were determined on the mushroom (Sarcodon aspratus) extracts in order to find out new food functional components. The antioxidative activities of water and ethanol extracts from the Sarcodon aspratus were measured by peroxide values (POV), electron?donating ability (EDA) using 1,1-diphenyl-2-picryl hydroxyl (DPPH), nitrite-scavenging ability and superoxide dismutase-like activity (SODA) by pyrogallol. The antioxidative activitiy of the ethanol extract measured by POV was higher than those of the water extract, BHT, and α-tocopherol. The EDA of the water extract and ethanol extract using DPPH showed the highest values of 76.94% and 73.06%, respectively. The nitrite-scavenging abilities (pH 1.2, 1,000 ppm) of the water and ethanol extracts were 72.61% and 62.69%, respectively, and the nitrite-scavenging ability of the water extract was higher than that of the ethanol extract in all pH values. The SODA of the ethanol extract was higher than that of the water extract. The Sarcodon aspratus extracts had antimicrobial effects on Listeria monocytogenes and Staphylococcus aureus.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

1 "Uric acid provides an antioxidant defense in humans against oxidant and radical-caused aging and cancer" 78 : 6858-6862, 1981

2 "The striatins-new antibiotics from the basidiomycete Cyathus striatus" 221-225, 1977

3 "The nitrite scavenging and electron donating ability of phenol compounds" 28 : 232-239, 1996

4 "Studies on the physiological functionality of pine needle and mugwort extracts" 27 : 978-984, 1995

5 "Studies on the constituents of the higher fungi of Korea An antibiotic component of Cryptoporus volvatus" 1980

6 "Studies on antioxidants of Hypsizigus marmoreus Effects of Hypsizigus marmoreus for antioxidants activities of mice plasma" 117 : 623-628, 1997

7 "Structures and antioxidant activity of diketopiperazines isolated from the mushroom Saccodon aspratus" 48 : 93-97, 2005

8 "SOD 유사활성물질과 기능성식품의 개발" 7 : 41-49, 1994

9 "Oxygen radicals in the adult respiratory distress syndrome and in cerebral vascular damage" 173-187, 1985

10 "On the antioxidatives activities of crude drugs" 25 : 691-694, 1978

11 "Mimicking of superoxide dismutase activity by protein-bound polysaccharide of Coriolus versicolor QUEL and oxidative stress relief for cancer patients" (moto k) : 40-46, 1992

12 "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismmutase" 47 : 468-474, 1975

13 "Inhibition of nitrosamine formation by nondialyzable melanoidins" 51 : 1333-1338, 1987

14 "Identification of environmental chemicals causing mutation and cancer" 204 : 589-592, 1970

15 "Evaluation and selection of antioxidative activities of 80 collected and mated mushroom strains" 13 : 689-693, 2004

16 "Effects of water soluble extract of Ganoderma lucidum kale juice and sodium dextrothyroxine on hormone and lipid metabolism in hypercholesterolemic rats 1 Concentrations of triiodothyronine blood sugar and lipid composition in serum" 381-386, 1990

17 "Effects of varied mushroom on lipid compositions in dietary hypercholesterolemic rats" 21 : 131-135, 1992

18 "Effects of the substances extracted from dried mushroom by several organic solvents on the stability of fat" 150-154, 1983

19 "Antioxidative and nitrite-scavenging activities of edible mushrooms" 29 : 432-436, 1997

20 "Antioxidative and nitrite-scavenging activities of edible mushrooms" 29 : 432-436, 1997

21 "Antioxidative activity of the extracts from browned oak mushroom (Lentinus edodes) with unmarketable quality" 36 : 648-654, 2004

22 "Antioxidative activity of the anthocyanin pigments cyanidin 3-O-β-D-glucoside and cyanidin" 42 : 2407-2410, 1994

23 "Antioxidant determination by the use of a stable free radical" 181 : 1199-1200, 1958

24 "Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill" Food Chem in press. 2006

25 "Antioxidant activity of extracts from the Lespedeza bicolor" 12 : 75-79, 2005

26 "Antioxidant activity and total phenolic compounds of P. eryngii spp. extracts." 50 : 216-219, 2005

27 "Antioxidant activity and inhibitive effects on human leukemia cells of edible mushrooms extracts" 12 : 80-85, 2005

28 "Antifungal antibiotic from the mushroom Agrocybe aegerita" 590-603, 1992

29 "Antibiotics from basidiomycetes IX an antifungal antibiotic from Oudemansiella mucida" 1112-1117, 1979

30 "Antibiotics from Basidiomycetes XXXI An antifungal sesterterpenoid from Aleurodiscus mirabilis" 1989

31 "Antibiotic substances from basidiomycetes Ⅳ" 343-351, 1949

32 "A colorimetric microdetermination of peroxide value utilizing aluminium chloride as the catalyst" 15 : 965-967, 1980