Pyrene 및 NBD 형광체와 다이펩타이드를 이용한 금속 센서의 합성

다국어 초록 (Multilingual Abstract)

Dansyl fluorophore containing various fluorescent sensors have been used for monitoring metal ions through an internal charge-transfer (ICT) sytem. Pyrene fluorophore have been used in the design of ratiometric sensor because the self assembled complex of pyrene-conjugated sensors causes a distinctive change in a fluorescence emission spectrumon of the pyrene excimer emission. NBD(Nitro-2,1,3-benzoxadiazole) fluorophore is excited by visible light and this fluorophore was used for a chemosensor that showed colorimetric and flourecent responose to Zn(II) ions. Thus, NBD containing diamino and sulfonyl group was synthesized and was conjugated into the amino acid to synthesize visible light active chemosenors. Interestingly, NBD-Trp showed turn on response and colorimetric response to Hg(II) among tested metal ions, whereas NBD-Ala showed turn off response to Hg(II).

다국어 초록 (Multilingual Abstract)

The detection and quantification of heavy metal ions are important in many applications, including environmental monitoring, waste management, developmental biology, and clinical toxicology. As amino acid and peptides are water-soluble and biologically and environmentally compatible, a new chemosensor based on dipeptides are a promising route of development for monitoring heavy metal ions in aqueous system. We designed dipeptide containing Met, His and synthesized fluorescent peptide sensor containing pyrene fluorophore for monitoring heavy metal ions. The peptide sensor showed a response to Cu(II), Ag(I) and Hg(II). Specially, the peptide sensor detected Ag(I) and Hg(II) by ratiometric response and Cu(II) by turn off response. The binding affinity and binding stoichiometry of the sensor to the metal ions were characterized.

목차 (Table of Contents)

• Part ⅠDesign of fluorescent chemosensors based on dipeptide and pyrene fluorophore for monitoring heavy metal ions in aqueous media 1
• Abstract (English) 2
• Abstract (Korea) 3
• 1. Introduction 4
• 1.1 Advantage of peptide chemosensor 4
• 2. Materials and Methods 6
• 2.1 Materials 6
• 2.2 Synthesis of Fluorescent Dipeptide Probe 6
• 2.3 Fluorescent Experiments 10
• 3. Results 11
• 3.1 Analysis of PMH 11
• 4. Conclusion 26
• 5. Reference 27
• Part II Synthesis of chemosensors for metal ions based on NBD as a fluorophore 28
• Abstract (English) 29
• Abstract (Korea) 30
• 1. Introduction 31
• 1.1 Design and Synthsis for Fluorescent Sensor 31
• 2. Materials and Methods 32
• 2.1 Materials 32
• 2.2 Synthesis of Fluorescent SBD probe 32
• 3. Results 37
• 3.1 Analysis of SBD probe 37
• 4. Conclusion 48
• 5. Reference 49