Curcumin의 간보호효과에 대한 연구 = Studies on the Hepatoprotective Effect of Curcumin

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the hepatoprotective effect of curcumin on acute hepatic damage and the antifibrotic effect on liver cirrhosis. The acute hepatic damage was induced by single dose of carbon tetrachloride (0.15ml/kg, i.p.). Mice were dosed 5Omg/kg or 200mg/kg(p.o.) of curcurnin dissolved in com oil for 4 consecutive days and 2 hours after the last treatment, carbon tetrachloride was injected. Serum ALT values in 50mg/kg or 200mg/kg dosed group, determined 24 hours after the injection of carbon tetrachloride, were decreased to 67% and 24%, respectively compared to those of carbon tetrachloride controled group. This result indicates that curcurnin has hepatoprotective effect on acute liver damage induced by carbon tetrachloride. N-terminal procollagen type III peptide(PIIIP) is known as on-going marker of liver fibrosis. To date, the determination of PIlIP in serum was performed by RIA. In this study, antibody was developed in rabbit using recombinant protein of human PIlIP as immunogen. This product showed cross reactivity with rat when fibrotic rat liver was immunostained with the antibody and it may be used in development of PIlIP detection in serum of experimental fibrotic rats by ELISA. And we also studied the antifibrotic effect of curcumin in cirrhotic rat. Liver cirrhosis was induced by dimethylnitrosamine (10 μ1/kg, i.p. for 3 consecutive days for 3 weeks). The rats were dosed 50mg/kg/day(p.o.) of curcumin for 3 weeks. Antifibrotic effects were evaluated by serum biochemical values, liver hydroxyproline contents, and light microscopical histology of the liver. The results indicated that liver cirrhosis was induced successfully in 3 weeks but curcumin had no antifibrotic effect on liver cirrhosis induced by dimethylnitrosamine.