Real-time PCR을 이용한 원유시료 유래 황색포도상구균의 신속 검출 = SYBR Green I-based Real-time PCR Assay and Melting Curve Analysis forRapid Detection of Staphylococcus aureus from Raw Milks Samples

다국어 초록 (Multilingual Abstract)

The aim of this study was to develop a LightCycler-based real time PCR (LC-PCR) assay and toevaluate its diagnostic use for the detection of Staphylococcus aureus in raw milk samples. Folowing amplificationof 113 bp of coa gene encoding an coagulase precursor specific for Staphylococcus aureus, melting curve and DNAsequencing analysis was performed to verify the specificity of the PCR products. Amplification of 209 bp gene encod-mecA), melting curve analysis and DNA sequencing analysis wasperformed to verify methicillin resistance Staphyloccus aureus (MRSA). According to this study, 6 of 647 raw milksamples showed S. aureus positive and 2 of them showed a mecA positive and the detection limit was 10 fg of DNA.And we also isolated Staphylococcus chromogenes a causative agent of exudative epidermitis in pigs and cattle from3 samples.

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