Ginseng γ-thionin is localized to cell wall-bound extracellular spaces and responsive to biotic and abiotic stresses

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P><P>Genomic DNA sequence of a <I>γ-thionin</I> in the <I>Panax ginseng</I> plant, termed <I>Pgγ-thionin</I>, contains 2 exons coding a 225-bp open reading frame with its deduced 75 amino acid sequence interrupted by 1 internal intron. The mRNA transcript level of <I>Pgγ</I>-thionin was ubiquitously expressed in the leaf, flower bud, stem, root and rhizome, with its highest expression in the roots. <I>Pgγ-thionin</I> was responsive to both biotic and abiotic stresses, such as an 18-fold increase after exposure to ABA. Fluorescent tagged-<I>Pgγ</I>-thionin protein was localized to cell wall-bound extracellular spaces. Our finding indicates that <I>Pgγ</I>-thionin might be involved in cellular defense responses.</P> <P><B>Graphical abstract</B></P><P><ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► We found the genomic DNA of γ-thionins from <I>P. ginseng</I> encodes 225 bp ORF. ► The 3-D structure of Pgγ-thionin is similar to the previously reported γ-thionin. ► <I>Pgγ-thionin</I> was responsive to sets of biotic and abiotic stresses. ► Pgγ-thionin was found to be localized to cell wall-bound extracellular spaces.</P>