In-House PCR법을 이용한 HLA-B27 형별 검사의 유용성 검토;PCR 검사 키트와 In-House PCR의 일치율 비교 = Utility of In-House PCR for HLA-B27 Typing: Comparison of Concordance Ratebetween PCR Kit and In-House PCR

다국어 초록 (Multilingual Abstract)

Background : Commercial kits of PCR method are widely used in HLA-B27 typing; however, their
cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing
it with that of a commercial kit.
Methods : HLA-B27 typing was done in 188 patients by using two PCR methods, AbsoluteTM HLAB27
PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house
method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System
9600 (Perkin-Elmer Centus Corp., USA).
Results : The commercial kit and in-house PCR showed 100% concordance rate with each other
in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative
by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%).
Conclusions : The in-house PCR is a reliable and cost-effective method and can replace or supplement
commercial kits for HLA-B27 typing. (Korean J Lab Med 2008;28:239-43)

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