Sambucus sieboldiana (SS) is a member of the familyCaprifoliaceae and has been recommended as a functionalmaterial because of its several bioactivities. Althoughnumerous literatures are available on the pharmacological andbiological activities, the bi...
Sambucus sieboldiana (SS) is a member of the familyCaprifoliaceae and has been recommended as a functionalmaterial because of its several bioactivities. Althoughnumerous literatures are available on the pharmacological andbiological activities, the biological activity of SS in boneregeneration process has not yet been well-defined. Therefore,in this study, the effect of SS was investigated in the proliferationand differentiation of MC3T3-E1 osteoblastic cell line. Thetreatment of SS did not significantly affect the cell proliferationin MC3T3-E1 cells. SS significantly accelerated themineralization and significantly increased the expression ofalkaline phosphatase (ALP) and osteocalcin (OC) mRNAs,compared to the control, in the differentiation of MC3T3-E1cells. SS significantly accelerated the decrease of osteonectin(ON) mRNA expression as compared with the control in atime-dependent manner in the differentiation of MC3T3-E1cells. These results suggest that the SS facilitate the osteoblastdifferentiation and mineralization in MC3T3-E1 osteoblastic cells. Therefore, there may be potential properties fordevelopment and clinical application of bone regenerationmaterials.