딸기 시들음병균에 대한 Trichoderma 속 균의 길항작용에 관한 연구 : Ⅲ. Chitinase and β-1,3-glucanase Activity Ⅲ. Chitinase와 β-1,3-glucanase 활성 = Studies on Antagonism of Trichoderma Species to Fusarium oxysporum f.sp.fragariae

국문 초록 (Abstract)

T. harzianum은 합성 배지에 유일한 탄소원으로서 chitin, laminarin, 본 병균의 세포벽, 밑기울 분말, clover잎 분말, maltose를 단독으로 첨가하여 생육시키면 chitinase와 β-1, 3-glucanase를 생성하였으나, chitinase 활성은 세포벽 첨가구에서 가장 높았고 다음은 chitin이었으며 maltose와 밑기울에서도 높은 활성을 보였으나 clover에서는 극히 낮았다. β-1, 3-glucanase 활성은 maltose에서 가장 높았고 다음은 laminarin이었으며 그 이외에는 극히 낮았다. 양 효소의 활성은 pH 3.5와 5.5가 pH 7.5에 비하여 높았으며, T. harzianum과 본 병균을 혼합하면 단독배양에 비하여 높았다. T. viride도 유일한 탄소원으로서 chitin 또는 laminarin을 첨가하면 각각 chitinase와 β-1,3-glucanase를 생성하였으나, chitinase 활성은 T. harzianum에 비하여 현저하게 낮았고 β-1,3-glucanase의 활성은 비슷하였다.

다국어 초록 (Multilingual Abstract)

Chitinase production by Trichoderma harzianum was highest in the culture with cell walls of the pathogen or chitin, moderately high with maltose or wheat bran, and very poor with clover leaf power. Relatively large amount of β-1,3-glucanase was excreted by T. harzianum when maltose or laminarin was used as a sole carbon source for the culture of the fungus, whereas cell walls, wheat bran and clover leaf powder had little effect on the enzyme production. For both of the enzymes, the enzyme activities were higher at pH 3.5-5.5 than at pH 7.5, and higher enzyme activities were detected in the dual culture of T. harzianum and Fusarium oxysporum f. sp. fragariae than in the culture of T. harzianum alone. The activity of chitinase by T. viride was significantly lower than by T. harzianum, and the activity of β-1,3-glucanase was similar between the two antagonisitcs, although T. viride produced chitinase and β-1,3-glucanase when chitin or laminarin was added as a sole carbon source.