FRTL-5 세포의 세포주기(Cell Cycle)에 TSH 및 그레이브병 IgG가 미치는 영향에 관한 Flow Cytometry를 이용한 분석 = Effect of TSH and Gravea IgG on the S - phase percent of FRTL - 5 Cells Determined by Flow Cytometry

다국어 초록 (Multilingual Abstract)

Controversy exists in the question that Graves' disease TSI (Thyroid stimulating immunoglobuin) and TGI (Thyroid growth immunoglobulin) coexist as different molecules or are the properties of a single IgG and current methods measuring TGI activity had been criticized, We tried to measure TGI activitty using FRTL-5 cells by flow ytometric analysis. The results were as follows.
1) Cell cycle analysis of FRTL-5 cells cultured without hormones (5% CS) showed 85.5±0.5% cells in Go and GI phase, 8.6±0.4% cells in S phase and 5.4±0.2% cells in G2M phase. When cultured with TSH(6 hormone medium), cells were 64.0±1.1% in Go and GI phase, 26.2±0.7% in S phase and 9.8±1.2% in G2M phase which means increased cell division.
2) When cultured with untreated Graves' IgG, the cell cycle analysis showed variable patterns. S phase percent in patients with goiter volume more than 40 ml was increasd (GII, 11.96±2.70 %) when compared with that of the patients with goiter volume less than 40 ml(GI, 9.90±2.57%). Overall, i8) of GI and 43%(7/16) of GII, Graves' IgG increased S phase percents when compared with normal IgG.
These resluts suggested that flow cytometric analysis of the cell cycle might be a valuable method of TGI assay providing direct measurements of cell division.(J Kor Soc Endocrinol 7:228~233.1992)