Efficient in vitro rooting system of shoot cuttings in chrysanthemum was developed. After 1 or 2 weeks in culture, the rooting of shoots cultured on 1/4MS medium were obtained and influenced largely by IBA and sucrose concentration. In IBA treatments ...
Efficient in vitro rooting system of shoot cuttings in chrysanthemum was developed. After 1 or 2 weeks in culture, the rooting of shoots cultured on 1/4MS medium were obtained and influenced largely by IBA and sucrose concentration. In IBA treatments rooting percentage and the number of roots per explants were increased in proportion to IBA concentration. The rooting percentage reached up to 100% in 0.1mg/L IBA. Without sucrose poor rooting was achieved, it showed that the addition of sucrose to medium was needed for successful rooting. The rooting percentage, the number of roots per explant and root length were enhanced with the addition of sucrose, and it was revealed that the optimal concentration of sucrose was 30g/L for desirable rooting. Conclusively the optimal medium for in vitro rooting was 1/4MS medium supplemented with 0.1mg/L IBA and 30g/L sucrose.