인간 유양돌기 골수 유래 간엽줄기세포의 획득 및 신경세포로의 분화 = Neural Differentiation of Mesenchymal Stem Cells from Bone Marrow of Human Mastoid Process

다국어 초록 (Multilingual Abstract)

Reports of neural differentiation of mesenchymal stem cells suggest the possibility that these
cells may serve as a source for stem cell-based regenerative medicine to treat neurological disorders. The purpose of this study
was to generate neural cells by differentiation of bone marrow-derived mesenchymal stem cells that isolated from human mastoid
process. Materials and Method：Human mesenchymal stem cells (hMSCs) isolated from human mastoid process bone marrow
during mastoidectomy for chronic otitis media surgery were characterized using fluorescence-activated cell sorter.
Induction of neural differentiation from hMSCs was performed using mitogenic factors (basic fibroblast growth factor, epidermal
growth factor, forskolin, isobutylmethylxanthine), and the characterization of differentiated hMSCs was performed using
immunohistochemistry, RT-PCR and whole cell patch clamp technique. Results：hMSCs from bone marrow of mastoid process
were isolated and cultured. Differentiated cells from hMSCs expressed mRNA transcripts for neuron specific markers, TUJ1 and
neurofilament proteins (NF-L, NF-M) as determined by RT-PCR, and neuron specific markers, suhc as NeuN, TUJ1, microtubule-
associated protein-2 (MAP2) and glial fibrillary acidic protein by immunohistochemistry. These cells showed voltagedependent
sodium currents that was blocked by tetrodotoxin. Conclusion：hMSCs, which were isolated from human mastoid
process bone marrow, were one of the good sources for stem cell-based regenerative medicine to treat neurological disorders.
(Korean J Otorhinolaryngol-Head Neck Surg 2008;51:422-8)

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