U-937 세포에서 세라마이드의 세포증식과 세포주기 조절단백질에 대한 작용 = Effect of Ceramide on Cell Growth and Cell Cycle Related Proteins in U-937 Cells

다국어 초록 (Multilingual Abstract)

Ceramide. a product of sphingomyelin hydrolysis, has been proposed as a lipid second messenger ymediating antiproliferative activation. In this study, we examined the role of the cell cycle-related proteins in the ceramide-mediated growth suppression. Treatment of U-937 cells with C2-ceramide(N-acetylsphingosine) resulted in growth suppression in a time- and concentration dependent manner. Ceramide induced concentration dependent dephosphorylation of retinoblastoma gene product (Rb). Rb remains hypophosphorylated in synchronized cells even after serum stimulation in the presence of ceramide. Ceramide decreased the expression of cyclin D1 and cyclin E levels. These results suggest that antiproliferative effect of ceramide is associated with hypophosphorylation of Rb and decreased expression of cyclin D1 and cyclin E.