Evaluation of the Agreement between Immunodot Assays and Intradermal Skin Testing or Favrot Diagnostic Criteria in Canine Atopic Dermatitis

다국어 초록 (Multilingual Abstract)

This study was undertaken to identify differences between atopic and non-atopic dogs in three rapid screeningimmunodot assays as well as the ability of the assays to predict the results of intradermal skin testing (IDST) or Favrotdiagnostic criteria (FDC). Twenty-nine dogs diagnosed with canine atopic dermatitis (CAD) were selected as the atopicgroup. Twenty-five dogs without CAD were included as the non-atopic group. Three types of immunodot assays wereconducted on all serum samples from both groups: Allercept E-screen 2nd generation (ES2G), Canine Allergic TendencyReference Test (ALERT), and Asan Easy Test Canine IgE (AETC). IDST, which included 39 allergens, and immunodotassays were performed concurrently in 13 dogs from the atopic group and compared. While there were no significantdifferences in positivity between the two groups in the evaluation of ALERT (P = 0.435) and AETC (P = 0.313),positivity in ES2G testing was significantly higher in the non-atopic group than the atopic group (P = 0.038). TheES2G, ALERT, and AETC results showed fair (κ = 0.235), slight (κ = 0.133), and slight (κ = 0.014) accordance withIDST, respectively. The outcomes of ES2G, ALERT, and AETC indicated poor (κ = −0.211), slight (κ = 0.106), andslight (κ = 0.087) agreement with FDC. In conclusion, rapid screening immunodot assays were not useful for thediagnosis of CAD. These assays may provide a supplementary method for predicting the results of IDST in atopic dogs.

참고문헌 (Reference)

1 Landis JR, "The measurement of observer agreement for categorical data" 33 : 159-174, 1977

2 Olivry T, "The ACVD task force on canine atopic dermatitis: forewords and lexicon" 81 : 143-146, 2001

3 DeBoer DJ, "The ACVD task force on canine atopic dermatitis (XVII): intradermal testing" 81 : 289-304, 2001

4 DeBoer DJ, "The ACVD task force on canine atopic dermatitis (XVI): laboratory evaluation of dogs with atopic dermatitis with serum-based “allergy” tests" 81 : 277-287, 2001

5 Diesel A, "Serum allergen-specific immunoglobulin E in atopic and healthy cats: comparison of a rapid screening immunoassay and complete-panel analysis" 22 : 39-45, 2011

6 Halliwell R, "Revised nomenclature for veterinary allergy" 114 : 207-208, 2006

7 Olivry T, "New diagnostic criteria for canine atopic dermatitis" 21 : 124-127, 2010

8 German AJ, "Measurement of IgG, IgM and IgA concentrations in canine serum, saliva, tears and bile" 64 : 107-121, 1998

9 Halliwell REW, "Induced and spontaneous IgE antibodies to Dermatophagoides farinae in dogs and cats: evidence of functional heterogeneity of IgE" 9 : 179-184, 1998

10 Olivry T, "Evaluation of the agreement between allergen specific intradermal or IgE serological tests and a point-ofcare immunodot assay in dogs with atopic dermatitis" 22 : 284-285, 2011

11 Hill PB, "Concentrations of total serum IgE, IgA, and IgG in atopic and parasitized dogs" 44 : 105-113, 1995

12 Park S, "Comparison of response to immunotherapy by intradermal skin test and antigen-specific IgE in canine atopy" 62 : 983-988, 2000

13 이예슬, "Comparison of rapid screening immunoassay and intradermal test for canine atopic dermatitis" 동물의학연구소 16 (16): 115-120, 2015

14 Foster AP, "Comparison of intradermal and serum testing for allergen-specific IgE using a FcεRIα-based assay in atopic dogs in the UK" 93 : 51-60, 2003

15 Zunic M, "Comparison between immunodot tests and the intradermal skin test in atopic dogs" 9 : 201-206, 1998

16 김하정, "Common allergens of atopic dermatitis in dogs: comparative findings based on intradermal tests" 대한수의학회 12 (12): 287-290, 2011

17 Zur G, "Canine atopic dermatitis:a retrospective study of 266 cases examined at the University of California, Davis, 1992-1998. Part I. Clinical features and allergy testing results" 13 : 89-102, 2002

18 Le Roy L, "Canine atopic dermatitis diagnostic criteria:evaluation of four sets of published criteria among veterinary students" 42 : 79-84, 2015

19 Favrot C, "A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis" 21 : 23-31, 2010