The aim of this study is to develop an advanced method for regeneration of three dimensional (3D) tissue or organ. The multi ?-head tissue/organ building system (MtoBS) which can deposit two or more biomolecules and various synthetic biomaterials was ...
The aim of this study is to develop an advanced method for regeneration of three dimensional (3D) tissue or organ. The multi ?-head tissue/organ building system (MtoBS) which can deposit two or more biomolecules and various synthetic biomaterials was developed to regenerate heterogeneous tissue. The MtoBS possessing 6 heads was developed to build 3D pre-tissue in which structural, physiological, and anatomical factors were considered. Two heads are designed for synthetic biomaterials as a framework, another two are for cell dispensing, and the others are for protein dispensing. Feasibility of MtoBS for building 3D pre-tissue was shown by dispensing three different biomaterials into one structure. It was proved that position and volume control of two different hydrogel were controllable in the MtoBS. In conclusion, the MtoBS is expected to be a promising system for regeneration of heterogeneous tissue.