Agrobacterium rubi와 A. tumefaciens에 의한 국화 뿌리혹병

다국어 초록 (Multilingual Abstract)

Crown gall on lower stem and root of chrysanthemum(Dendranthema grandiflorum Kitamura) was observed
at Hwasung and Gumi in 2001 and 2004, respectively. Tumors were semi-round with rough surface texture of
dark brown color. Nine isolates inducing gall formation on lower stem of chrysanthemum among twenty isolates
from the tumor tissues were characterized. Their colonies were convex, glistening, circular with an
entire edge and whitish or tannish cream in color on potato dextrose agar supplemented with 0.5% CaCO3.
The virulent isolates were rod-shaped with peritrichous flagellae, gram negative, aerobic and growing on
D1M agar. Among nine virulent isolates, one isolate was identified as Agrobacterium rubi and eight isolates
were A. tumefaciens based on biochemical and physiological characteristics, fatty acid profiles and substrate
utilization patterns. A. tumefaciens had strong pathogenicity and broad host range compared with A. rubi.
This is the first report on crown gall of chrysanthemum in Korea. To our knowledge, crown gall of chrysanthemum
caused by A. rubi is first report in this study worldwide.

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