Anti - Progesterone Monoclonal Antibody 에 대한 Anti - Idiotype 항체의 불임효과에 관한 연구 = Antifertility Effect of Anti - Idiotypic Antibody Against Anti - Progesterone Monoclonal Antibody

다국어 초록 (Multilingual Abstract)

The active immunization of progesterone produces high titer antibodies. However, the progesterone itself binds to the receptor sites and produces the side effect on the contraceptive activities of the antibodies. Therefore, the anti-idiotypic antibody (anti-Id) was studied whether it had the same effect as the active immunization of progesterone to prevent pregnancy or not. If it has antifertility effect, it could be utilized as a contraceptive vaccine. The passive immunization of antibody to mice was previously studied and found that anti-progesterone monoclonal antibody prevents pregnancy by degenerating the embryos in the oviduct and uterus (Yoon et al., 1989). The polyclonal anti-Id was obtained by immunizing anti-progesterone monoclonal antibody (15A) to rabbit and then purified using affinity column chromatography. Furthermore, three different clones (G2, G9, H6) of monoclonal anti-Ids have been raised against anti-progesterone monoclonal antibody. The relationships between the antifertility effects and anti-progesterone antibody level were studied by the active immunization of anti-Id and progesterone-BSA. The results showed that a higher antifertility effect was observed with progesterone-BSA immunization (70%) than with anti-Id immunization (55%). The plasma level of anti-progesterone was as high as 3,500 ㎍/㎖ with progesterone-BSA immunization. The comparison study of the antibody levels between pregnant (10 ㎍/㎖) and non-pregnant mice (44 ㎍/㎖) after immunization with the anti-Id indicates that antibody formation at a certain level prevents pregnancy. However, the antibody level is not proportional to the antifertility effect in mice.