毒素生産大腸菌의 生化學的性狀에 관한 硏究 = Studies on the Biochemical Characteristics of Enterotoxigenic Escherichia coli

다국어 초록 (Multilingual Abstract)

The pathogenesis of gastrointestinal infections caused by E. coli has been attributed to the ablility of some strains to produce enterotoxins or to invade the intestinal mucosa directly.
Enterotoxigenic strains may produce either a heat-labile toxin(LT), a heat-stable toxin(ST) or both LT and ST.
The heat-labile enterotoxin of E. coli causes a diarrhea with a delayed onset and similalar to cholera toxin in its mode of action and immunogenicity.
In contrast to LT, ST is of low molecular weight, nonimmunogenic, and not neutralized by cholera or LT antitoxin.
The infant mouse test was used for the detection of ST and EnzymeL-inked Immunosorbent Assay was used to dettect LT, and serological test was used to detect Enteropathogenic E. coli and Enteroinva sive E. coli.
And biochemical examinations were done.
1. Strains of pathogenic E. coli isolated from the diarrheal patients in Seoul were identifided in 68 (21.3%) of 319 cases.
2. Stranis of enterotoxigenic E. coli was detcetected in 56 cases; 49 were positive for heat-labile enterotoxin and 1 was positive for only the heat-stable enterotoxin.
3. Strains of enteropathogenic E. coli was detected in 10 cases.
4. The serotypes of enteropathogenic E. coli were 026 : K60, 055 : K59, 0111 : K58, 0119 : K69, 0126 K71, 0128 : K67, 01 : K51.
5. D-mannitol, lactose, maltose were used for growth in toxigenic E. coli.
Malonate utilization, urease activity, gelatin liquefaction were negetive in enteropathogenic E. coli, enterotoxigenic E. coli and enteroinvasive E. coli.