EQCN을 이용한 티타늄 소재의 생화학적 표면개질을 위한 SAMs 형성의 정량적 고찰 = Quantitative Analysis of SAMs Formation for Biochemical Surface Modification of Titanium Materials with EQCN

다국어 초록 (Multilingual Abstract)

Self-assembled monolayers(SAMs) have become a basic technique for biochemical modification using e.g. specific petides on a biomaterial surface. Some studies have been reported on the effect of their terminal functionalities of the orgarnic molecules on apatite formation in a simulated body fluid and the surface chemical characteristics of the SAMs infuenced the biological response.
In this study we assumed that if the alaknethiol or organosilane SAMs are formed on a titanium surface, such a surface might be used for an attachment site inducing biological functionality such as organo-functional group, peptide, protein, etc. And we examined the alkanethiol or organosilane adsorption behavior on the Au, c.p Ti and TiO² surfaces by using an electrochemical quartz crystal nanobalance(EQCN) developed in our laboratory. The resolution limit of the constructed EQCN was about 0.1 ng. Using the EQCN, we were able to precisely investigate the mass change ratio on the surfaces versus immersion time.
The amount of Thiol-I SAM adsorbed on c.p Ti was about 67% compared with that formed on the Au coated titanium substrate. However, there was no SAM formation on the TiO₂ surface with thick titanium oxide layer (obtained using the electrochemical oxidation method). In case of organosilanes, the amount of Silane-I SAM adsorbed on TiO₂ was about 19% more than c.p Ti substrate and Silane-II was 14%.
Results obtained from this study, have shown that by altering titanium surface chemistry, titanium surface may be modified into a functional bioactive surface by introducing functional groups and can be used in the same way as Au substrate.

목차 (Table of Contents)

• 목차
• 제1장 서론 = 1
• 제2장 연구배경 = 4
• 2.1 Ti의 자연 산화막 = 4
• 2.2 생화학적 표면개질법 = 5
• 2.3 SAMs(Self-Assembled Monolayers) = 6
• 2.4 EQCN(Electrochemical Quartz Crystal Nanobalance) = 7
• 제3장 연구목적 = 10
• 제4장 재료 및 방법 = 11
• 4.1 EQCN의 제작 = 11
• 4.1.1 시스템 구성 = 11
• 4.1.2 전원 = 11
• 4.1.3 I/O 인터페이스 카드 = 11
• 4.1.4 데이터 수집 장치 = 12
• 4.1.5 Potentiostat와 Oscillator = 13
• 4.1.6 EQCN 셀(Cell) = 13
• 4.1.7 Software 및 구동환경 = 14
• 4.2 전극 = 14
• 4.3 시약 = 14
• 4.4 기능기(Functional Group)를 이용한 SAMs 형성 = 15
• 4.5 전기화학적 방법에 의한 TiO₂표면 형성 = 16
• 4.5.1 AFM(Atomic Force Microscopy) = 17
• 4.5.2 SAES(Scanning Auger Electron Spectroscopy) = 18
• 제5장 결과 및 고찰 = 20
• 5.1 EQCN의 성능평가 = 20
• 5.2 각 전극 표면에서 Thiol기에 대한 SAMs 형성 = 22
• 5.3 각 전극 표면에서 Silane기에 대한 SAMs 형성 = 26
• 5.4 Thiol, Silane기에 대한 SAMs 형성정도 비교 = 30
• 제6장 결론 = 35
• 참고문헌 = 36
• 부록 APPLICATION OF EQCN FOR QUANTITATIVE ANALYSIS OF ALKANETHIOL ADSORBED ON MODIFIED Ti SURFACES = 39