In Vitro and In Vivo Effects of Two Coconut Oils in Comparison to Monolaurin on Staphylococcus aureus: Rodent Studies

다국어 초록 (Multilingual Abstract)

Since monolaurin, a monoglyceride formed in the human body in small quantities, has proven effective both in vitro and in vivo against certain strains of Staphylococcus aureus, an important question arises whether consuming a substance high in lauric acid content, such as coconut oil could increase intrinsic monolaurin production to levels that would be successful in overcoming staphylococcal and other microbial invaders. Both a cup plate method and a microdilution broth culture system were employed to test bacteriostatic and bactericidal effects of the test agents in vitro. To test effectiveness in vivo, female C3H/he mice (10–12 per group) were orally administered sterile saline (regular control), vancomycin (positive control), aqueous monolaurin, or two varieties of coconut oil (refined, bleached, deodorized coconut oil and virgin coconut oil) for 1 week before bacterial challenge and 30 days after. A final group received both monolaurin and vancomycin. In contrast to monolaurin, the coconut oils did not show bactericidal activity in vitro. In vivo, the groups receiving vancomycin, monolaurin, or the combination showed some protection—50–70% survival, whereas the protection from the coconut oils were virtually the same as control—0–16% survival. Although we did not find that the two coconut oils are helpful to overcome S. aureus infections, we corroborated earlier studies showing the ability of monolaurin to do such.

참고문헌 (Reference)

1 Shilhavy B, "What is Virgin Coconut Oil?"

2 Preuss HG, "The potential for developing natural antibiotics: examining oregano and monolaurin" 12 : 119-124, 2005

3 Brandt Rose S, "Studies with the agar cup-plate method. 1. A standardized agar cup plate technique" 38 : 525-537, 1939

4 Freireich EJ, "Quantitative comparison of toxicity of anticancer agents in mouse, rat, hamster, dog, monkey, and man" 50 : 219-244, 1966

5 Preuss HG, "Minimum inhibitory concentrations of herbal essential oils and monolaurin for gram positive and negative bacteria" 272 : 29-34, 2005

6 Enig MG, "Lauric oils as antimicrobial agents: theory of effect, scientific rationale, and dietary applications as adjunct nutritional support for HIV-infected individuals, In Nutrients and Foods in AIDS" CRC Press 81-97, 1998

7 Kabara JJ, "Inhibition of Staphylococcus aureus, In The Pharmacological Effect of Lipids II" American Oil Chemists’ Society 71-75, 1985

8 Andrade RJ, "Hepatic safety of antibiotics used in primary care" 66 : 1431-1446, 2011

9 Kabara JJ, "Health oils from the tree of life (nutritional and health aspects of coconut oil)" 31 : 2-8, 2000

10 Kabara JJ, "Fatty acids and derivatives as antimicrobial agents–a review, In The Pharmacological Effects of Lipids" American Oil Chemists’ Society 1-14, 1978

11 Kabara JJ, "Fatty acids and derivatives as antimicrobial agents" 2 : 23-28, 1972

12 Enig MG, "Enig on a therapeutic dose"

13 Preuss HG, "Effects of essential oils and monolaurin on Staphylococcus aureus: in vitro and in vivo studies" 15 : 279-285, 2005

14 Reagan-Shaw S, "Dose translation from animal to human studies revisited" 22 : 659-661, 2008

15 Gould IM, "Coping with antibiotic resistance: the impending crisis" 36 (36): S1-S2, 2010

16 Boucher HW, "Bad bugs, no drugs, no ESKAPE! An update from the Infectious Diseases Society of America" 48 : 1-12, 2009

17 Manohar V, "Antifungal activities of origanum oil against Candida albicans" 228 : 111-117, 2001

18 Novotny J, "Adverse drug reactions to antibiotics and major antibiotics drug interactions" 18 (18): 126-139, 1999

19 Lambert RJ, "A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol, and carvacrol" 91 : 453-462, 2001

20 Lieberman S, "A review of monolaurin and lauric acid: natural virucidal and bactericidal agents" 12 : 310-314, 2006