Berberine Ameliorates Cold and Mechanical Allodynia in a Rat Model of Diabetic Neuropathy

다국어 초록 (Multilingual Abstract)

This study evaluated the antiallodynic properties of berberine on cold and mechanical allodynia after streptozotocin (STZ)-induced diabetes using a rat model. Diabetic neuropathy was induced in rats by intraperitoneal injection of STZ. To measure cold and mechanical allodynia, a 4 C plate and von Frey filament were used, respectively. Cold and mechanical allodynia induced by diabetes were significantly decreased by single and repeated intraperitoneal treatment of amitriptyline at 10 mg/kg, and berberine at 10 and 20 mg/kg. The hepatic malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase activities were significantly increased in diabetic rats as compared with those in intact rats; however, in amitriptyline- and berberine-treated rats, they were significantly decreased as compared to the STZ control. The overall effects of berberine 20 mg/kg on cold and mechanical allodynia were quite similar to those of amitriptyline 10 mg/kg, and berberine exhibited similar antioxidant effects as the same dosage of amitriptyline. In conclusion, berberine (10 and 20 mg/kg) was observed to have antiallodynic effects against diabetes, which are presumed to be associated with antioxidative effects. It can be considered that the anti-inflammatory or antidepressant capacity of berberine could contribute to the antiallonynic effects shown in this study.

참고문헌 (Reference)

1 Davidson E, "The roles of streptozotocin neurotoxicity and neutral endopeptidase in murine experimental diabetic neuropathy" 2009 : 431980-, 2009

2 Das Evcimen N, "The role of protein kinase C activation and the vascular complications of diabetes" 55 : 498-510, 2007

3 Ozkaya YG, "The effect of exercise on brain antioxidant status of diabetic rats" 28 : 377-384, 2002

4 Jasmin L, "The cold plate as a test of nociceptive behaviors: description and application to the study of chronic neuropathic and inflammatory pain models" 75 : 367-382, 1998

5 Vincent AM, "The antioxidant response as a drug target in diabetic neuropathy" 9 : 94-100, 2008

6 Paglia DE, "Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase" 70 : 158-169, 1967

7 Zurek JR, "Spinal and supraspinal components of opioid antinociception in streptozotocin induced diabetic neuropathy in rats" 90 : 57-63, 2001

8 Baynes JW, "Role of oxidative stress in development of complications in diabetes" 40 : 405-412, 1991

9 Wada R, "Role of advanced glycation end products and their receptors in development of diabetic neuropathy" 1043 : 598-604, 2005

10 Remppis A, "Rhizoma Coptidis inhibits LPS-induced MCP-1/CCL2 production in murine macrophages via an AP-1 and NFkappaB-dependent pathway" 2010 : 194896-, 2010

11 Morello CM, "Randomized double-blind study comparing the efficacy of gabapentin with amitriptyline on diabetic peripheral neuropathy pain" 159 : 1931-1937, 1999

12 Yilmaz HR, "Protective effect of caffeic acid phenethyl ester (CAPE) on lipid peroxidation and antioxidant enzymes in diabetic rat liver" 18 : 234-238, 2004

13 Cameron NE, "Pro-inflammatory mechanisms in diabetic neuropathy: focus on the nuclear factor kappa B pathway" 9 : 60-67, 2008

14 Imanshahidi M, "Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine" 22 : 999-1012, 2008

15 Pieper GM, "Peroxidative stress in diabetic blood vessels. Reversal by pancreatic islet transplantation" 44 : 884-889, 1995

16 Tesfaye S, "Painful diabetic peripheral neuropathy: consensus recommendations on diagnosis, assessment and management" 27 : 629-638, 2011

17 Vincent AM, "Oxidative stress in the pathogenesis of diabetic neuropathy" 25 : 612-628, 2004

18 Kulkarni SK, "On the mechanism of antidepressantlike action of berberine chloride" 589 : 163-172, 2008

19 Love A, "Nerve function and regeneration in diabetic and galactosaemic rats: antioxidant and metal chelator effects" 314 : 33-39, 1996

20 Kong LD, "Monoamine oxidase inhibitors from rhizoma of Coptis chinensis" 67 : 74-76, 2001

21 Pabreja K, "Minocycline attenuates the development of diabetic neuropathic pain: possible anti-inflammatory and anti-oxidant mechanisms" 661 : 15-21, 2011

22 Figueroa-Romero C, "Mechanisms of disease: the oxidative stress theory of diabetic neuropathy" 9 : 301-314, 2008

23 Draper HH, "Malondialdehyde determination as index of lipid peroxidation" 186 : 421-431, 1990

24 Kobayashi H, "MMPs initiate Schwann cell-mediated MBP degradation and mechanical nociception after nerve damage" 39 : 619-627, 2008

25 Talbot S, "Key role for spinal dorsal horn microglial kinin B1 receptor in early diabetic pain neuropathy" 7 : 36-, 2010

26 Kumar A, "JSH-23 targets nuclear factorkappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence" 13 : 750-758, 2011

27 Takeshita N, "Insulin attenuates formalin-induced nociceptive response in mice through a mechanism that is deranged by diabetes mellitus" 281 : 315-321, 1997

28 Berrocoso E, "Evaluation of milnacipran, in comparison with amitriptyline, on cold and mechanical allodynia in a rat model of neuropathic pain" 655 : 46-51, 2011

29 Aksoy N, "Effects of melatonin on oxidative-antioxidative status of tissues in streptozotocin-induced diabetic rats" 21 : 121-125, 2003

30 Reaven P, "Effect of streptozotocin-induced hyperglycemia on lipid profiles, formation of advanced glycation endproducts in lesions, and extent of atherosclerosis in LDL receptor-deficient mice" 17 : 2250-2256, 1997

31 Giugliano D, "Diabetes mellitus, hypertension, and cardiovascular disease: which role for oxidative stress?" 44 : 363-368, 1995

32 Xie W, "Diabetes is an inflammatory disease: evidence from traditional Chinese medicines" 13 : 289-301, 2011

33 Santini SA, "Defective plasma antioxidant defenses and enhanced susceptibility to lipid peroxidation in uncomplicated IDDM" 46 : 1853-1858, 1997

34 Kong WJ, "Combination of simvastatin with berberine improves the lipid-lowering efficacy" 57 : 1029-1037, 2008

35 Aebi H, "Catalase in vitro" 105 : 121-126, 1984

36 Kolosov A, "CNSB004 (Leconotide) causes antihyperalgesia without side effects when given intravenously: a comparison with ziconotide in a rat model of diabetic neuropathic pain" 11 : 262-273, 2010

37 Xu MG, "Berberineinduced mobilization of circulating endothelial progenitor cells improves human small artery elasticity" 22 : 389-393, 2008

38 Lee YS, "Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states" 55 : 2256-2264, 2006

39 Jeong HW, "Berberine suppresses proinflammatory responses through AMPK activation in macrophages" 296 : E955-E964, 2009

40 Jia L, "Berberine suppresses amyloid-betainduced inflammatory response in microglia by inhibiting nuclear factor-kappaB and mitogen-activated protein kinase signalling pathways" 64 : 1510-1521, 2012

41 Kong W, "Berberine is a novel cholesterollowering drug working through a unique mechanism distinct from statins" 10 : 1344-1351, 2004

42 Chen FL, "Berberine inhibits the expression of TNFalpha, MCP-1, and IL-6 in AcLDL-stimulated macrophages through PPARgamma pathway" 33 : 331-337, 2008

43 Meng S, "Berberine ameliorates inflammation in patients with acute coronary syndrome following percutaneous coronary intervention" 39 : 406-411, 2012

44 Morrow TJ, "Animal models of painful diabetic neuropathy: the STZ rat model" 9 (9): 1-11, 2004

45 Wang C, "Ameliorative effect of berberine on endothelial dysfunction in diabetic rats induced by high-fat diet and streptozotocin" 620 : 131-137, 2009

46 Cho SY, "Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocininduced diabetic rats by supplementation of dandelion water extract" 317 : 109-117, 2002

47 Vallianou N, "Alpha-lipoic Acid and diabetic neuropathy" 6 : 230-236, 2009

48 Oates PJ, "Aldose reductase, still a compelling target for diabetic neuropathy" 9 : 14-36, 2008

49 Durak I, "A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction" 214 : 103-104, 1993