모기 살충성 내독소를 생산하는 Bacillus thuringiensis subsp. guiyangiensis 21-2균주(H serotype 43)의 특성 = Characterization of a Mosquitocidal Delta-endotoxin from Bacillus thuringiensis subsp. guiyangiensis strain 21-2(H serotype 43)

다국어 초록 (Multilingual Abstract)

To prevent appearance of resistant mosquitoes against δ-endotoxin of Bacillus thuringiensis subsp. israelensis (Bu) in field, a mosquitocidal Bacillus thuringiensis strain 21-2(Bt 21-2) producing a new type of δ-endotoxin was isolated. The strain Bt 21-2 belongs to H serotype43. B. thuringiensis subsp. guiyangiensis(Btg). The δ-endotoxins from the strain Bt 21-2 and the strain Bti were a cuboid shape morphologically, but the δ-endotoxin from the type strain Btg was a bipyramidal shape on phase contrast microscope. The δ-endotoxin of the strain Bt 21-2 was composed of 150.90 and 70 KDa proteins on SDS-PAGE, and the antigenicity of δ-endotoxin of the strain Bt 21-2 was different from that of the strain Bti on immunoblot. The δ-endotoxin gene of the strain Bt 21-2 was not amplified with specific primers of δ-endotoxin gene (cry4A and cry4B) of the strain Bti on PCR.