Screening for Korean Vegetables with Anticarcinogenic Enzyme Inducing Activity Using Cell Culture System

다국어 초록 (Multilingual Abstract)

There is extensive evidence suggesting the protective role of fruits and vegetables against chemically induced carcinogenesis. We have tested the ability of a representative range of Korean vegetables to act as blocking agents against neoplastic initiation by determining the induction level of quinone reductase, an anticarcinogenic marker enzyme, in hepa1c1c7 cells exposed to vegetable extracts. Among thirty vegetables tested, Arctium lappa (Burdock), Brassica juncea (Mustard leaf), Pteridium aguilinum (Bracken) and Chrysanthemum coronarium (Crown daisy) caused a significant induction of quinone reductase activity with a limited increase in arylhydrocarbon hydroxylase activity. Combination of crown daisy with burdock had synergistic effect on quinone reductase induction. Quinone reductase-inducing activity was found mostly in hexane and ethylacetate fractions of MeOH extract of crown daisy while it was not detected in n-butanol and water fractions. Animal study using SD rats demonstrated that crown daisy intake induced quinone reductase activity in liver, kidney, lung, and small intestine, confirming the presence of potent QR inducer(s) in crown daisy. These data suggest that some vegetables including crown daisy induced QR merits further investigation as a potential cancer preventive agent in human.

목차 (Table of Contents)

• Abstract
  
• INTRODUCTION
  
• MATERIALS AND METHODS
  
• RESULTS AND DISCUSSION
  
• ACKNOWLEDGEMENTS
  
• REFERENCES