오크라 분말의 Quercetin-3-o-Gentiobioside 및 Isoquercitrin의 분석법 개선 및 검증

다국어 초록 (Multilingual Abstract)

This study aimed to investigate the validation and modify the analytical method to determine quercetin- 3-o-gentiobioside and isoquercitrin in Abelmoschus esculentus L. Moench for the standardization of ingredients in development of functional health products. The analytical method was validated based on the ICH (International Conference for Harmonization) guidelines to verify the reliability and validity there of on the specificity, linearity, accuracy, precision, detection limit and quantification limit. For the HPLC analysis method, the peak retention time of the index component of the standard solution and the peak retention time of the index component of A. esculentus L. Moench powder sample were consistent with the spectra thereof, confirming the specificity. The calibration curves of quercetin-3-o-gentiobioside and isoquercitrin showed a linearity with a near-one correlation coefficient (0.9999 and 0.9999), indicating the high suitability thereof for the analysis. A. esculentus L. Moench powder sample of a known concentration were prepared with low, medium, and high concentrations of standard substances and were calculated for the precision and accuracy. The precision of quercetin-3-o-gentiobioside and isoquercitrin was confirmed for intraday and daily. As a result, the intra-day precision was found to be 0.50-1.48% and 0.77-2.87%, and the daily precision to be 0.07-3.37% and 0.58-1.37%, implying an excellent precision at level below 5%. As a result of accuracy measurement, the intra-day accuracy of quercetin-3-o-gentiobioside and isoquercitrin was found to be 104.87-109.64% and the daily accuracy thereof was found to be 106.85-109.06%, reflecting high level of accuracy. The detection limits of quercetin-3-o-gentiobioside and isoquercitrin were 0.24 μg/mL and 0.16 μg/mL, respectively, whereas the quantitation limits were 0.71 μg/mL and 0.49 μg/mL, confirming that detection was valid at the low concentrations as well. From the analysis, the established analytical method was proven to be excellent with high level of results from the verification on the specificity, linearity, precision, accuracy, detection limit and quantitation limit thereof. In addition, as a result of analyzing the content of A. esculentus L. Moench powder samples using a validated analytical method, quercetin-3-o-gentiobioside was analyzed to contain 1.49±0.01 mg/dry weight g, while isoquercitrin contained 1.39±0.01 mg/dry weight g. The study was conducted to verify that the simultaneous analysis on quercetin-3-o-gentiobioside and isoquercitrin, the indicators of A. esculentus L. Moench, is a scientifically reliable and suitable analytical method.

목차 (Table of Contents)

• ABSTRACT
• Materials and Methods
• 실험재료 및 시약
• Quercetin-3-o-gentiobioside 및 isoquercitrin 분석
• 표준용액 및 시험용액의 조제
• 지표성분 분석법 유효성 검증
• 특이성
• 직선성
• 정밀성 및 정확성
• 검출한계 및 정량한계
• Results and Discussion
• Quercetin-3-o-gentiobioside 및 isoquercitrin의 크로마토그램
• 특이성 확인
• 검량선을 이용한 직선성 확인
• 정밀성 및 정확성
• 검출한계 및 정량한계
• 오크라 분말의 quercetin-3-o-gentiobioside 및 isoquercitrin함량분석
• Acknowledgement
• 국문요약
• Conflict of interests
• ORCID
• References