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      KCI등재 SCIE SCOPUS

      Surface Display of Lipolytic Enzyme, Lipase A and Lipase B of Bacillus subtilis on the Bacillus subtilis Spore

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      https://www.riss.kr/link?id=A105096674

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      다국어 초록 (Multilingual Abstract)

      For the enhancement of lipase stability in organic solvent containing reaction, live immobilization method, using Bacillus subtilis spore as a display vehicle was attempted. Bacillus subtilis coat protein cotE was used as an anchoring motif for the display of lipA and lipB of Bacillus subtilis. Using this motif, lipolytic enzyme Lipase A and Lipase B were functionally displayed on the surface of Bacillus subtilis spore. Purified spore displaying CotELipB fusion protein showed higher lipolytic activity compared to that of CotE-LipA fusion protein. The surface localization of Lipase B was verified with flow cytometry and protease accessibility experiment. Spore displayed lipase retained its activity against acetone and benzene which completely deactivated free soluble lipase in the same reaction condition.
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      For the enhancement of lipase stability in organic solvent containing reaction, live immobilization method, using Bacillus subtilis spore as a display vehicle was attempted. Bacillus subtilis coat protein cotE was used as an anchoring motif for the di...

      For the enhancement of lipase stability in organic solvent containing reaction, live immobilization method, using Bacillus subtilis spore as a display vehicle was attempted. Bacillus subtilis coat protein cotE was used as an anchoring motif for the display of lipA and lipB of Bacillus subtilis. Using this motif, lipolytic enzyme Lipase A and Lipase B were functionally displayed on the surface of Bacillus subtilis spore. Purified spore displaying CotELipB fusion protein showed higher lipolytic activity compared to that of CotE-LipA fusion protein. The surface localization of Lipase B was verified with flow cytometry and protease accessibility experiment. Spore displayed lipase retained its activity against acetone and benzene which completely deactivated free soluble lipase in the same reaction condition.

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      참고문헌 (Reference)

      1 Boder, E. T., "Yeast surface display for screening combinatorial polypeptide libraries" 15 : 553-558, 1997

      2 Kwon, S. J., "Transgalactosylation in a water-solvent biphasic reaction system with beta-galactosidase displayed on the surfaces of Bacillus subtilis spores" 73 : 2251-2256, 2007

      3 Van Pouderoyen, G., "The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme" 309 : 215-212, 2001

      4 Hosseini-Abari, A., "Surface display of bacterial tyrosinase on spores of Bacillus subtilis using CotE as an anchor protein" 56 : 1331-1337, 2016

      5 Jung, H. C., "Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae" 16 : 576-580, 1998

      6 Kim, J. H., "Spore-displayed streptavidin: A live diagnostic tool in biotechnology." 331 : 210-214, 2005

      7 Washida, M., "Spacer-mediated display of active lipase on the yeast cell surface" 56 : 681-686, 2001

      8 Kim, J. H., "Method for expression of proteins on spore surface"

      9 Eggert, T., "Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure" 502 : 89-92, 2001

      10 Kumar, A., "Lipase catalysis in organic solvents: Advantages and applications" 18 : 2-, 2016

      1 Boder, E. T., "Yeast surface display for screening combinatorial polypeptide libraries" 15 : 553-558, 1997

      2 Kwon, S. J., "Transgalactosylation in a water-solvent biphasic reaction system with beta-galactosidase displayed on the surfaces of Bacillus subtilis spores" 73 : 2251-2256, 2007

      3 Van Pouderoyen, G., "The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme" 309 : 215-212, 2001

      4 Hosseini-Abari, A., "Surface display of bacterial tyrosinase on spores of Bacillus subtilis using CotE as an anchor protein" 56 : 1331-1337, 2016

      5 Jung, H. C., "Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae" 16 : 576-580, 1998

      6 Kim, J. H., "Spore-displayed streptavidin: A live diagnostic tool in biotechnology." 331 : 210-214, 2005

      7 Washida, M., "Spacer-mediated display of active lipase on the yeast cell surface" 56 : 681-686, 2001

      8 Kim, J. H., "Method for expression of proteins on spore surface"

      9 Eggert, T., "Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure" 502 : 89-92, 2001

      10 Kumar, A., "Lipase catalysis in organic solvents: Advantages and applications" 18 : 2-, 2016

      11 Cowan, D., "Industrial enzyme technology" 14 : 177-178, 1996

      12 Schreuder, M. P., "Immobilizing proteins on the surface of yeast cells" 14 : 115-120, 1996

      13 Li, L., "Functional display of foreign protein on surface of Escherichia coli using N-terminal domain of ice nucleation protein" 85 : 214-221, 2004

      14 Hwang, B. Y., "Functional display of active tetrameric β-galactosidase using Bacillus subtilis spore display system" 13 : 2313-2319, 2013

      15 Ueda, M., "Establishment of cell surface engineering and its development" 80 : 1243-1253, 2016

      16 Sousa C., "Enhanced metalloadsorption of bacterial cells displaying poly-His peptides" 14 : 1017-1020, 1994

      17 Richter, A., "Disulfide bonds of proteins displayed on spores of Bacillus subtilis can occur spontaneously" 71 : 156-161, 2015

      18 Kim, J. H., "Display of proteins on Bacillus subtilis endospores" 66 : 3127-3136, 2009

      19 Georgiou G., "Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines" 15 : 29-34, 1997

      20 Lee, S. H., "Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysis" 70 : 5074-5080, 2004

      21 Haima, P., "Development of a beta-galactosidase alphacomplementation system for molecular cloning in Bacillus subtilis" 86 : 63-69, 1990

      22 Dartois V., "Cloning, nucleotide sequence and expression in Escherichia coli of a lipase gene from Bacillus subtilis 168" 1131 : 253-260, 1992

      23 Richins, R. D., "Biodegradation of organophosphorus pesticides by surfaceexpressed organophosphorus hydrolase" 15 : 984-987, 1997

      24 Hwang, B. Y., "Bacterial surface display of co-factor containing enzyme, w-transaminase from Vibrio fluvialis using Bacillus subtilis spore display system" 75 : 1862-1865, 2011

      25 KIM, JUNE-HYUNG, "Bacterial Surface Display of GFPUV on Bacillus subtilis Spores" 한국미생물·생명공학회 17 (17): 677-680, 2007

      26 Kobayashi, G., "Accumulation of an artificial cell wallbinding lipase by Bacillus subtilis wprA and/or sigD mutants" 188 : 165-169, 2000

      27 Eggert T., "A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase" 267 : 6459-6469, 2000

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      학술지 이력
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      2023 평가예정 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
      2020-01-01 평가 등재학술지 유지 (해외등재 학술지 평가) KCI등재
      2011-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2009-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2007-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2004-01-01 평가 등재학술지 선정 (등재후보2차) KCI등재
      2003-01-01 평가 등재후보 1차 PASS (등재후보1차) KCI등재후보
      2001-07-01 평가 등재후보학술지 선정 (신규평가) KCI등재후보
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      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 1.14 0.13 0.75
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      0.57 0.46 0.239 0.02
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