오이 발효음료가 만성적으로 에탄올을 급여한 흰쥐의 에탄올 대사와 항산화방어계에 미치는 영향

국문 초록 (Abstract)

본 연구는 다량으로 폐기되는 오이를 이용하기 위하여 개발한 오이 발효원액을 주원료로 제조한 숙취해소 음료의 간보호 효능을 검증하기 위하여 만성적으로 에탄올을 섭취시킨 흰쥐에서 에탄올 대사, 항산화 방어계, 간독성 관련지표 및 지질함량 변화를 살펴보았다. 실험동물은 4 주령의 수컷 SD계 흰쥐 24마리를 1주간 고형식이로 적응시킨 후 난괴법에 의하여 에탄올대조군(Control) 및 에탄올 섭취 흰쥐에게 헛개열매 추출물을 주원료로 하여 개발한 숙취해소 물질인 SKM 급여군(SKM) 또는 SKM을 함유한 오이 발효음료 급여군(CF＋SKM)으로 나누었다. SKM과 CF＋SKM은 사람의 하루 섭취량을 기준으로 체중 ㎏당 7 ㎖씩 매일 일정시각에 경구투여 하였다. SKM과 CF＋SKM은 체중과 식이섭취에는 영향을 미치지 않았으며, CF＋SKM군의 신장무게가 대조군보다 낮았다. 혈장 중 에탄올 함량은 대조군에 비하여 CF＋SKM군에서 유의적으로(p＜0.05) 낮았으며, SKM군은 낮은 경향을 보였다. 혈장 중의 아세트알데히드 함량은 대조군에 비하여 SKM과 CF＋SKM군 모두 각각 40.6%와 48.4% 유의적인(p＜0.05) 개선 효과를 보였다. 간조직 중의 ADH 활성은 실험군간 유의적인 변화가 없었으나 CYP2E1 활성은 SKM과 CF＋SKM 모두 대조군에 비하여 유의적으로 (p＜0.05) 낮았다. 간조직의 CYP2E1 활성은 혈장 중의 아세트알데히드 함량과 양의 상관관계(r=0.566, p＜0.01)였다. 간조직의 ALDH 활성은 SKM과 CF＋SKM 모두 대조군에 비하여 유의적으로(p＜0.05) 높았으며 혈장의 아세트알데히드 농도와 유의적 음의 상관관계(r=-0.564, p＜0.01)를 보였다. SKM군과 CF＋SKM군의 간조직내 SOD와 CAT 활성과 GSH 함량이 대조군에 비하여 유의적으로 높았다. 반면, SKM과 CF＋SKM은 간조직 중의 지질과산화물 생성을 대조군에 비하여 각각 유의적으로 낮추었다. SKM과 CF＋SKM 급여 시 에탄올대조군에 비하여 각각 AST 활성은 29%와 44% 낮았으며, ALT 활성은 42%와 34% 낮았다. 혈장의 총 콜레스테롤과 간조직의 콜레스테롤 함량은 대조군에 비하여 SKM과 CF＋SKM군에서 유의적으로(p＜0.05) 낮았으며 특히, CF＋SKM의 간조직내 중성지질 함량은 대조군에 비하여 유의적으로(p＜0.05) 낮았다. SKM군과 CF＋SKM군의 간조직 중 지방축적이 대조군에 비하여 감소되었다. 이와 같이 SKM과 CF＋SKM은 간조직의 CYP2E1 활성을 억제하고 ALDH 활성과 항산화 방어계를 향상시킴으로써 에탄올로 인한 간독성을 보호할 수 있을 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

Cucumber fermentation has been used as a means of preservation. This study was performed to investigate the effects of fermented cucumber beverage (CF) containing beneficial materials for an ethanol hangover based on Hovenia dulcis (SKM) on ethanol-induced hepatotoxicity. Male Sprague-Dawley rats were randomly divided into three groups: ethanol control, ethanol plus SKM, and ethanol plus CF＋SKM. SKM or CF＋SKM was orally administered at a dose of 7 ㎖/㎏ body weight once per day for 5 weeks. Control rats were given an equal amount of water. CF＋SKM significantly lowered plasma ethanol levels, whereas SKM tended to decrease the levels compared to the control. Both SKM and CF＋SKM significantly lowered the plasma acetaldehyde levels and serum transaminase activities compared to those in the control. SKM and CF＋SKM did not affect hepatic alcohol dehydrogenase activity; however, it significantly inhibited cytochrome P450 2E1 (CYP2E1) activity. Hepatic aldehyde dehydrogenase (ALDH) activity was significantly higher in the SKM and CF＋SKM groups than that in the control group. Plasma acetaldehyde concentration was significantly correlated with hepatic CYP2E1 (r=0.566, p＜0.01) activity and ALDH (r=-0.564, p＜0.01) activity. Hepatic superoxide dismutase and catalase activities as well as glutathione content increased with the SKM and CF＋SKM administration, whereas lipid peroxide content decreased significantly. Furthermore, SKM and CF＋SKM lowered plasma and hepatic lipid content and lipid droplets compared to those in the control group. These results indicate that SKM and CF+SKM exhibit hepatoprotective properties partly by inhibiting CYP2E1 activity, enhancing ALDH activity and stimulating the antioxidant defense systems in ethanol-treated rats.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 글
• 문헌

참고문헌 (Reference)

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