참나물이 고콜레스테롤식이를 섭취한 흰쥐의 지질대사에 미치는 영향

국문 초록 (Abstract)

참나물 에탄올 추출물이 고콜레스테롤식이로 유발된 고콜레스테롤혈증 억제효과를 검토한 결과 고콜레스테롤식이만을 급여한 HC군은 정상식이만을 급여한 NC군에 비하여 높은 체중증가량을 나타내었다. 고콜레스테롤식이와 참나물 에탄올 추출물을 저용량과 고용량 병합투여한 HC-PBL군과 HC-PBH군은 HC군에 비하여 체중증가량이 저하되었으나 유의차는 없었다. 식이섭취량은 HC군이 다른 실험군들에 비하여 유의하게 저하되었다. 식이효율은 고콜레스테롤식이를 급여한 HC군, HC-PBL군 및 HC-PBH군은 NC군에 비하여 높았다. 체중 당 간 무게는 HC군이 다른 모든 실험군들에 비하여 유의하게 증가되었으나, 참나물 에탄올 추출물 병합투여로 체중 당 간 무게가 저하되었으며 NC군보다는 높게 나타났다. 고콜레스테롤식이 급여로 증가된 혈청 중 AST, ALT 및 ALP 활성은 참나물 에탄올 추출물 병합투여로 활성이 감소되었으며, 참나물 에탄올 추출물 투여 용량이 증가할수록 농도 의존적으로 감소하였다. 혈청 중 지질 함량에서, 고콜레스테롤식이를 급여한 HC군은 정상식이를 급여한 NC군에 비하여 중성지질과 총콜레스테롤 함량이 유의하게 증가되었고, 고콜레스테롤식이와 참나물 에탄올 추출물을 병합투여로 감소되었으나 고용량 병합투여한 HC-PBH군만 유의하게 저하되었다. 혈청 중 인지질 함량은 HC군이 NC군에 비하여 감소하였고 참나물 에탄올 추출물 투여로 증가되었으나 NC군에 비하여는 낮았다. 혈청 중 유리콜레스테롤과 콜레스테릴 에스테르 함량은 고콜레스테롤식이와 참나물 에탄올 추출물을 병합투여한 HCPBL군과 HC-PBH군은 HC군에 비하여 감소하였으며, 참나물 에탄올 추출물 투여 용량에 따른 유의차가 없었다. 총콜레스테롤에 대한 콜레스테롤 에스테르 비율은 각 실험군들 간에 유의차가 없었다. 혈청 중 LDL-콜레스테롤 함량은 HC군이 NC군에 비하여 유의하게 증가하였다. 고콜레스테롤식이와 참나물 에탄올 추출물을 병합투여한 HC-PBL군과 HC-PBH군은 HC군에 비하여 LDL-콜레스테롤 함량이 저하되었으나 참나물 에탄올 추출물 투여 용량에 따른 차이는 없었다. HC-PBL군과 HC-PBH군의 혈청 중 HDL-콜레스테롤 함량은 HC군에 비하여 증가하였으며, 심혈관위험지수와 동맥경화지수는 유의하게 저하되었으나 NC군 수준에는 미치지 못하였다. 간 중 중성지질 및 총콜레스테롤 함량은 HC군이 NC군에 비하여 유의하게 증가되었고, 고콜레스테롤식이와 참나물 에탄올 추출물을 병합투여한 HC-PBL군과 HC-PBH군은 HC군에 비하여 참나물 에탄올 추출물 투여로 저하되었으나 NC군보다는 높게 나타났다. 간 중 중성 지질 함량은 참나물 에탄올 추출물 투여 용량에 따른 유의차가 없었으나, 총콜레스테롤 함량은 고용량 병합투여한 HC-PBH군만 유의하게 저하되었다. 혈청 및 간 중의 지질함량 변화는 정상식이를 급여한 NC군과 NC-PB군 간에는 유의차가 없었다. 따라서 고콜레스테롤식이를 급여하면서 참나물 에탄올 추출물을 병합투여 시에만 지질대사 개선 효과가 있는 것으로 여겨지며, 고용량 병합투여 시 효능이 더 큰 것으로 나타났다. 고콜레스테롤식이로 인한 산화적 스트레스가 고콜레스테롤혈증을 유발하였으며, 이는 참나물 에탄올 추출물에 함유된 항산화물질을 포함한 여러 생리활성 물질이 영향을 미친 것으로 사료된다. 이상의 실험 결과에서 참나물 에탄올 추출물은 고콜레스테롤식이로 증가된 총콜레스테롤, LDL-콜레스테롤 및 중성지질 함량은 감소시켰고, HDL-콜레스테롤과 인지질 함량은 증가시킴으로써 지방간 및 동맥경화의 예방과 치료에 효과적일 것으로 판단되어 진다.

다국어 초록 (Multilingual Abstract)

This study was performed to investigate the effect of ethanol extract of Pimpinella brachycarpa (PB) on serum and liver lipid metabolism in rats. Male Sprague-Dawley rats were administered 1% cholesterol and 0.25% sodium cholate to induce hypercholesterolemia. PB ethanol extract (200 ㎎/㎏/day or 400 ㎎/㎏/day) was also administered orally to rats with high cholesterol diet for 6 weeks. We divided 40 rats into five groups; normal diet group (NC), high cholesterol diet group (HC), normal diet and PB ethanol extract (200 ㎎/㎏) administered group (NC-PB), high cholesterol diet and PB ethanol extract (200 ㎎/㎏) administered group (HC-PBL), and high cholesterol diet and PB ethanol extract (400 ㎎/㎏) administered group (HC-PBH). The growth rate and liver weight of the high cholesterol diet group was higher than those of the normal diet group, whereas those of the groups administered PB ethanol extract were gradually decreased. There was a signigicant increase in the activities of serum alanine aminotransferase (ALT), asparate aminotransferase (AST) and alkaline phosphatase (ALP) in the high cholesterol diet group. The administration of PB ethanol extract decreased serum ALT, AST and ALP activities in a dose-dependent manners. The high cholesterol diet group showed increased serum triglyceride, total cholesterol, free cholesterol and LDL-cholesterol levels, and decreased atherogenic index, HDL-cholesterol and phospholipid levels as compared with the normal diet group. PB ethanol extract administrated groups showed increased HDL-C/T-C, HDL-cholesterol and phospholipid levels, and decreased serum triglyceride, total cholesterol, free cholesterol, and LDL-cholesterol levels as compared with the high cholesterol diet group. There were no differences in the concentrations of serum triglyceride, phopholipid, LDL-cholesterol, HDL-cholesterol and free cholesterol between normal diet groups. The hepatic concentrations of total cholesterol and triglyceride were also lower in PB ethanol extract administrated groups than in the high cholesterol diet group. These results suggest that ethanol extract of PB exerts hypocholesterolemic effect by reducing serum and liver cholesterol contents.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

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