Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel

다국어 초록 (Multilingual Abstract)

Chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogels were rapidly and stably prepared without any crosslinking materials by using an engineered tyrosinase (mTyr-CNK) with high catalytic activity for tyrosine/ DOPA-tethered polymeric biomaterials throughout a broad pH range. A dual-barrel syringe with one part containing chitosan/mTyr-CNK solution and the other containing gelatin solution with/without nanohydroxyapatite was successfully used to form homogeneous hydrogels at room temperature followed by 37 oC to simulate an in situ injection approach. The obtained hydrogels exhibited an average pore size greater than 150 m and high swelling ratios with similar mechanical properties to other chemically crosslinked chitosan/gelatin hydrogels. The in vitro degradation properties and cellular viability suggested that the hydrogels could be used as biodegradable and biocompatible scaffolds for biomedical applications, such as space filling biomaterials and delivery vehicles for bioactive molecules and cells. These results demonstrated that mTyr-CNK-mediated hydrogels have remarkable promise as an injectable scaffold biomaterial.

참고문헌 (Reference)

1 L. Fan, 146 : 427-, 2016

2 M. Liu, 5 : 17014-, 2017

3 H. Jiankang, 5 : 453-, 2009

4 K. S. Anseth, 17 : 1647-, 1996

5 J. Patterson, 13 : 14-, 2010

6 Y. L. Li, 41 : 2193-, 2012

7 R. LogithKumar, 151 : 172-, 2016

8 L. D. Zhu, 132 : 38-, 2018

9 K. Su, 37 : 2139-, 2015

10 Y. J. Yang, 13 : 1800086-, 2018

11 L. Cui, 99 : 31-, 2014

12 S. M. Saraiva, 5 : 63478-, 2015

13 M. Nieto-Suarez, 141 : 175-, 2016

14 Y. R. Choi, 129 : 50-, 2018

15 X. Bai, 3 : 401-, 2018

16 M. Otsuka, 36 : 1653-, 2013

17 C. Ohtsuki, 6 : S349-, 2009

18 H. Zhou, 7 : 2769-, 2011

19 R. Z. LeGeros, 14 : 65-, 1993

20 S. I. Stupp, 26 : 169-, 1992

21 H. Do, 7 : 17267-, 2017

22 R. N. Raja Mohd Hafidz, 18 : 787-, 2011

23 C. Q. Yan, 39 : 3528-, 2010

24 L. Guo, 36 : 10009-, 2003

25 S. F. Yan, 7 : 17005-, 2017

26 M. I. Gariboldi, 3 : 151-, 2015

27 김은해, "Preparation of in situ Injectable Chitosan/Gelatin Hydrogel Using an Acid-tolerant Tyrosinase" 한국생물공학회 23 (23): 500-506, 2018

28 Yan-yan Pei, "Hydrogels with diffusion-facilitated porous network for improved adsorption performance" 한국화학공학회 35 (35): 2384-2393, 2018

29 Soyeon Jo, "Development of Cellulose Hydrogel Microspheres for Lipase Immobilization" 한국생물공학회 24 (24): 145-154, 2019