Diversity of Endophytic Enterobacteria Associated with Different Host Plants

다국어 초록 (Multilingual Abstract)

Fifty-three endophytic enterobacteria isolates from citrus, cocoa, eucalyptus, soybean, and sugar cane were
evaluated for susceptibility to the antibiotics ampicillin and kanamycin, and cellulase production. Susceptibility
was found on both tested antibiotics. However, in the case of ampicillin susceptibility changed according
to the host plant, while all isolates were susceptible to kanamycin. Cellulase production also changed
according to host plants. The diversity of these isolates was estimated by employing BOX-PCR genomic
fingerprints and 16S rDNA sequencing. In total, twenty-three distinct operational taxonomic units (OTUs)
were identified by employing a criterion of 60% fingerprint similarity as a surrogate for an OTU. The 23
OTUs belong to the Pantoea and Enterobacter genera, while their high diversity could be an indication of
paraphyletic classification. Isolates representing nine different OTUs belong to Pantoea agglomerans, P.
ananatis, P. stewartii, Enterobacter sp., and E. homaechei. The results of this study suggest that plant species
may select endophytic bacterial genotypes. It has also become apparent that a review of the Pantoea/
Enterobacter genera may be necessary.

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