키토산 처리가 콩나물의 Soyasaponin 함량변화에 미치는 효과 = Effects of Chitosan Treatment on Changes of Soyasaponin Contents in Soybean Sprouts

국문 초록 (Abstract)

키토산을 농도별로 처리하여 재배한 콩나물의 조사포닌은 대조구에 비해 모두 키토산을 처리한 콩나물에서 더 많이 증가하였다. 생장기간 별로 키토산을 처리했을 때, 생장 $5{\sim}6$일째 조사포닌 함량이 가장 높았으며 대조구, 2,000, 1,000, 500 ppm 그리고 250 ppm 농도로 키토산을 처리한 콩나물 순서로 조사포닌 함량이 증가하였다가 7일째에는 감소하였다. 키토산을 농도별로 처리한 콩나물에서 그룹 B 사포닌의 함량을 분석하였을 때 soyasaponin I 은 250 ppm 처리구 콩나물에서 가장 높았으며 더 높은 농도로 처리했을 때에는 키토산의 농도에 따라서 함량이 감소하였다. Soyasaponin II의 함량은 대조구 콩나물보다 키토산 처리구 콩나물이 키토산의 농도를 증가시켜 처리하였을 때 증가하여 1,000 ppm을 처리했을 때 최고값을 보이다가 더 높은 농도인 2,000ppm을 처리했을 때에는 감소하였다. 하지만 콩나물 재배중 키토산 처리는 soyasaponin V의 함량변화에는 거의 영향을 미치지 않았다. Sovasaponin III과 IV의 함량은 soyasaponin II와 비슷하게 키토산을 1,000 ppm 처리할 때까지 농도에 따라 증가하였으나 유의적인 차이는 아니었으며 2,000 ppm 키토산 처리구에서는 약간 감소하였다.

다국어 초록 (Multilingual Abstract)

Elicitors are defined as substances that induce defense responses in plants, which include an increased synthesis of secondary metabolites. Saponin, one of the secondary metabolites, has various physiological effects such as anticancer, antioxidant, cholesterol-lowering activities, etc, in human. This study was carried out to find whether a treatment of soybean sprouts with chitosan as an elicitor, increases saponin contents. Saponin contents in soybean sprouts increased by the chitosan treatment during cultivation, reached the peak on the sixth day, and then decreased. A biosynthesis of group B soyasaponin appeared to be regulated differently. The content of soyasaponin I, a member of group B saponin, was the highest in 250 ppm chitosan-treated soybean sprouts, while the contents of soyasaponin II, III and IV were the highest in 1,000 ppm chitosan-treated soybean sprouts. The content of soyasaponin V changed little in soybean sprouts that had been treated with various concentration of chitosan.

참고문헌 (Reference)

1 오봉윤, "콩나물 생장 중 사포닌의 변화" 한국식품과학회 35 (35): 1039-1044, 2003

2 Park IK, "Sugar and free amino acid content of chitosan-treated soybean sprouts" 8 : 105-110, 2003

3 Osbourn AE, "Saponins and plant defence-A soap story" 1 : 4-9, 1996

4 Jung CY, "SPSSWIN for Statistics Analysis. Version 10.0" Muyok Publishing Co 276-283, 2002

5 Yoshiki Y, "Relationship between chemical structure and biological activities of triterpenoid saponin from soybean" 62 : 2291-2299, 1998

6 Darvill AG, "Phytoalexins and their elicitors-A defense against microbial infection in plants" 35 : 243-275, 1984

7 Cote F, "Oligosaccharide elicitors in host-pathogen interactions generation. perception and signal transduction, In Subcellular biochemistry: plant microbe interactions" Plenum Publishing Co 385-432, 1998

8 Michael W, "Modes of action of defensive secondary metabolites, In Functions of plant secondary metabolites and their exploitation in biotechnology" Sheffield Academic Press 17-133, 2000

9 Fuzzati N, "Identification of soyasaponin by liquid chromatography-thermospray mass spectrometry" 777 : 233-238, 1997

10 Morrissey JP, "Fungal resistance to plant antibiotics as a mechanism of pathogenesis" 63 : 708-724, 1999

11 Cho JE, "Enhanced resistance of soybean sprout by elicitor treatment" 2002

12 Oh BY., "Effects of elicitor treatment on changes of saponin content in soybean sprout during cultivation" Mokpo National University 2004

13 Lee YS, "Effect of chitosan treat-ment on growig characteristics of soybean sprouts" 31 : 153-157, 1999

14 Kim HJ, "Effect of chitosan on biological properties of sweet basil(Ocimum basilicum L. )" 53 : 3696-3701, 2005

15 Kim JH, "Current status and future prospects for development of plant defense activatiors" 14 : 15-19, 2001

16 Benhamou N., "Chitosan-mediated induced resistance: a promising strategy for plant disease management" Mokpo National University, Mokpo. p 135-151 135-151, 1999

17 No HK, "Chitosan treatment affects yield, ascorbic acid content, and hardness of soybean sprouts" 68 : 680-685, 2003

18 Reserch committee on textbook of natural products chemistry, "Chemistry of organic natural products" Younglim 369-, 1989

19 Lee YS, "Changes of free sugars, lipoxygenase activity and effects of chitosan treatment during cultivation of soybean sprouts" 31 : 115-121, 1999