Increased Expression of the TGF-β Isoform and Changed Contents of Collagen in Tendon of Cerebral Palsy Patients

국문 초록 (Abstract)

목적: 뇌성마비 환자의 아킬레스건 내 TGF-β isoform의 발현 정도와 콜라겐 구성을 알고자 하였다.
대상 및 방법: 뇌성마비로 인한 경직성 첨족마비 환자 중 수술적 연장술을 시행하였던 15명 환자의 아킬레스건 중앙부를 약 2×2×5 ㎣ 크기로 채취한 후, 면역조직화학 염색과 RT-PCR을 이용하여 TGF-β와 콜라겐 구성을 조사하였다.
결과: 아킬레스건 중앙부의 구성은 섬유모세포와 콜라겐 섬유 등의 기질로 구성되어 있었다. 면역조직화학 염색상 TGF-β1과 TGF-β2는 15예 중 각각 2예, 4예에서만 발현되었으며, TGF-β3의 경우는 7예에서 약하게 발현되었다. 경직성이 심한 비보행성 마비를 보인 7예 중 6예에서 TGF-β3의 양성 소견을 보였다. 콜라겐 염색상 경직성이 심한 비보행성 마비 환자에서는 상대적으로 미성숙형태인 제3형의 콜라겐이 증가됨을 알 수 있었다. 면역조직화학 염색 및 RT-PCR상 TGF-β3가 중등도 이상 발현된 7예에서 제3형 콜라겐이 증가되어 있었다.
결론: 뇌성마비로 인한 지속적인 근 구축은 건내 콜라겐 성분의 변화를 초래하고 이 과정중 TGF- 가 관여할 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

Purpose: This study measured the expression level of the transforming growth factor-β(TGF-β) isoform expression and the collagen composition within the Achilles tendon from cerebral palsy (CP) patients.
Materials and Methods: The Achilles tendons were obtained from 15 CP patients with spastic equinovarus. The presence of the TGF-β isoform and the composition of the collagen were examined histologically, performing by immunohistochemical staining (IHS) and determining the mRNA expression level using a reverse transcriptase polymerase chain reaction (RT-PCR).
Results: IHS revealed the presence of TGF-β1 and TGF-β2 expression in 2/15 cases and 4/15 cases respectively, and weak TGF-β3 expression in 7/15 cases. The TGF-β1 and TGF-β2 expression levels were uniform in all 15 cases according to RT-PCR, while TGF-β3 expression was observed in 8 out of 15 cases. IHS and RT-PCR showed strong TGF-β3 expression in 6/7 non-ambulatory patients. An immature form of collagen, type Ⅲ collagen, was observed more abundantly in the non-ambulatory patients.
Conclusion: These results suggest that contracture in CP may induce changes in the type of collagen via growth factors such as TGF-β.

목차 (Table of Contents)

• MATERIALS AND METHODS
• RESULTS
• DISCUSSION
• CONCLUSION
• REFERENCES

참고문헌 (Reference)

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