AStudy on Optimization of Marigold Petal Yield, Pure Lutein, and Formulation of Free-Flowing Lutein Esters

다국어 초록 (Multilingual Abstract)

The present study was conducted with the main objective to optimize petal yield from important marigold cultivars in WestBengal, and to standardize isolation of lutein from petals because these dietary xanthophylls are known to reduce the risks of agerelatedmacular degeneration (AMD) and cataracts. Six cultivars were studied viz. African marigold-Double (AFM-D), Africanmarigold-Single (AFM-S), African marigold-Orange (AFM-O), French marigold-Orange (FRM-O), French marigold-Double (FRMD),and LC (Local type), which withstand typical environmental conditions of northern West Bengal and produce flowers that do notvary in color and are relatively unaffected by pests and diseases. Lutein esters were extracted from milled marigold petals using nhexane.
However, overall performance showed that LC is superior to the other cultivars. Pure lutein was obtained after saponificationwith 50% KOH. The re-crystallized lutein was characterized by UV-VIS, IR spectroscopy, and HPLC. A free-flowing lutein esterwas also formulated. This free-flowing lutein ester was found to be suitable to undergo commercialization or subsequent processing.
An economic method for petal yield and isolation of lutein was thus standardized

참고문헌 (Reference)

1 Rice-Evans CA, "Why do we expect carotenoids to be antioxidants in vivo?" 26 : 381-398, 1997

2 Wong TY, "The epidemiology of age-related eye diseases in Asia" 90 : 506-511, 2006

3 Tian Y, "The effect of lutein supplementation on blood plasma levels of complement factor D, C5a and C3d" 8 : e73387-, 2013

4 Larsen E, "Simple saponication method for the quantitative determination of carotenoids in green vegetable" 50 : 1071-1072, 2002

5 Oliver J, "Semi-quantification of carotenoids by high performance liquid chromatorgraphy:saponification-induced losses in fatty foods" 829 : 393-399, 1998

6 Khachik F., "Process for isolation, purification, and recrystallization of lutein from saponified marigolds oleoresin and uses thereof"

7 Nirmalan PK, "Prevalence of vitreoretinal disorders in a rural population of Southern India: The Aravind Comprehensive Eye Study" 122 : 581-586, 2004

8 Izumi-Nagai K, "Macular pigment lutein is anti-inflammatory in preventing choroidal neovascularization" 27 : 2555-2562, 2007

9 Landrum JT, "Lutein, zeaxanthin and macular pigment" 385 : 28-40, 2001

10 Muriach M, "Lutein effect on retina and hippocampus of diabetic mice" 41 : 979-984, 2006

11 Jin XH, "Inhibitory effects of lutein on endotoxin-induced uveitis in Lewis rats" 47 : 2562-2568, 2006

12 Khachik F, "Identification of lutein and zeaxanthin oxidation products in human and monkey retinas" 38 : 1802-1811, 1997

13 Nishita KD, "Grinding methods: their impact on rice flour properties" 59 : 46-49, 1982

14 Sommerburg O, "Fruits and vegetables that are sources of lutein and zeaxanthin: the macular pigment in human eye" 82 : 907-910, 1998

15 Verghese J, "Focus on xanthophylls from Tagetes erecta L. the giant natural complex-I" 33 : 8-13, 1998

16 Chew BP, "Effects of lutein from marigold extract on immunity and growth of mammary tumors in mice" 16 : 3689-3694, 1996

17 Li SY, "Effect of lutein on retinal neurons and oxidative stress in a model of acute retinal ischemia/reperfusion" 50 : 836-843, 2009

18 Breithaupt DE, "Differentiation between lutein monoester regioisomers and detection of lutein diesters from marigold flowers (Tagetes erecta L.)and several fruits by liquid chromatography-mass spectrometry" 50 : 66-70, 2002

19 Akay C, "Could rice be used as an anticaking agent in table salt?" 60 : 95-99, 2009

20 Mangels AR, "Carotenoid content of fruits and vegetables: an evaluation of analytic data" 93 : 284-296, 1993

21 Semba RD, "Are lutein and zeaxanthin conditionally essential nutrients for eye health?" 61 : 465-472, 2003

22 Chen XJ, "Application of near-infrared reflectance spectroscopy to evaluate the lutein and β-carotene in Chinese kale" 22 : 148-153, 2009

23 Sujith PAA, "A Study on the different methods of preparation of lutein from supercritical fluid processed lutein esters" 2 : 154-, 2012