식염이 구강세균의 산 생성에 미치는 영향에 관한 연구 = Effects of Salt on Acid Production of Oral Bacteria

다국어 초록 (Multilingual Abstract)

The purpose of this study was to investigate the effect of salt on acid production in sucrose solution by oral bacteria. Streptococcus mutans 10449, OMZ 65, OMZ 175 were mixed and cultured in brain-heart infusin broth for 18 hours. Dental plaque and saliva were collected from a male orthodontic patient, ten-year-old, of high caries activity and cultured by the same method. S.mutans, plaque bacteria, and saliva bacteria were used as inocula, and the effects of varying concentrations of NaCl on acid production in sucrose solution was observed in vitro.
1. 10% salt inhibited acid production in 5% sucrose solution by S.mutans.
2. 7% slat inhibited acid production in 5%, 10%, and 20% sucrose solutions with 1% tryptose by oral bactera, especilly S.mutans.
3. 5% salt inhibited acid production in 5% sucrose solution with 0.4% beef extract and 0.04% bromo-cresol green by oral bacteria, espicially S.mutans, and the effect of NaCl and NaF were synergistic.
4. Four minute exposure of bacteria to 12.5% salt inhibited acid production in 40% sucrose solution with 2% tryptose, 0.5% NaCl, and 0.004% bromocresol green by oral bacteria, especially S.mutans, and the effect was greater than that of 0.1% NaF, and the effects of NaCl and NaF were synergistic.
In summary, salt had 'inhibitory effect on acid production in sucrose solution by oral bacteria, the effects of NaCl and NaF were synergistic, and four minute exposure to concentrated salt solution also had inhibitory effect.