TiO2 나노튜브 크기에 따른 미생물의 부착과 생장에 미치는 효과 = The Effect of TiO2 Nanotube Size on Microbial Adherence and Growth

국문 초록 (Abstract)

본 연구의 목적은 TiO2 나노튜브의 크기가 여러 병원성미생물의 생장과 생장에 미치는 영향을 살펴보는 것이다. 사용한 미생물은 그람음성균, Acinetobacter baumannii, Pseudomonas aeruginosa 와 그람양성균 Staphylococcus aureus이 며, 모두 병원에서 쉽게 감염이 되고, 치과나 정형외과의 임플란트 수술시 주입되는 여러 이식장치 주변에서 감염이 용이 하여, 치사율이 매우 높은 미생물들이다. 본 실험에서는, 4가지의 다른 크기(30 nm, 50 nm, 70 nm, 100 nm)의 TiO2 나 노튜브 표면위에서 같은 수의 각각의 미생물을 24시간동안 배양한 후, Scanning Electron Microscopy (SEM) 장비를 이 용하여 미생물의 부착정도를 살펴보았다. 대조구로서 Ti 로 코팅된 나노튜브를 사용하였다. 그 결과, 그람음성균 A. baumannii와 P. aeruginosa은 30 nm 크기의 TiO2 나노튜브에서는 대조구보다 부착능이 감소하였으나, 대체적으로 나노튜 브크기가 증가할수록 세균의 부착능이 향상되어, 100 nm 크기의 TiO2 나노튜브에서 세균부착능이 최대로 높았다. 하지만 이와 다르게, 그람양성균 S. aureus의 경우에는 TiO2 나노튜브 크기에 관계없이, 대조구에서만 월등히 부착능이 증가된 것을 확인하였다. 따라서, 본 연구에서는 그람음성균의 경우에 TiO2 나노튜브크기가 증가할수록 세균의 부착효과가 향상 되었고, 이에 반하여, 그람양성균은 TiO2에서보다 Ti에서의 부착능이 높다는 것을 확인하였다.

참고문헌 (Reference)

1 Breme, "Titanium and titanium alloys biomaterials ofpreference. Memoires et Etudes Scientifiques" 1989

2 Park J, "TiO2 nanotube surfaces: 15 nm-anoptimal length scale of surface topography for cell adhesion and differentiation" 2009

3 Puckett SD, "The relationship between the nanostructure of titanium surfaces and bacterial attachment" 2010

4 Shen XT, "Study of the correlation between Streptococcus mutans' adhesion and surface corrosion in casting titanium and Ti alloy" 2008

5 Tsuang YH, "Studies of photokilling of bacteria using titanium dioxide nanoparticles" 2008

6 Harris LG, "Staphylococci and implant surfaces: A review" 2006

7 Wickham JR, "Solid-state NMR studies of bacterial lipoteichoic acid adsorption on different surfaces" 2008

8 Oh S, "Significantly accelerated osteoblast cell growth onaligned TiO2 nanotubes" 2006

9 Ohko Y, "Self-sterilizingand self-cleaning of silicone catheters coated with TiO(2)photocatalyst thin films: a preclinical work" 2001

10 Henrichfreise B, "Resistance mechanisms of multiresistant Pseudomonas aeruginosa strains from Germany and correlation with hypermutation" 2007

11 Hetrick EM, "Reducing implant-related infections: Active release strategies" 2006

12 Maddikeri RR, "Reduced medical infection related bacterial strains adhesion on bioactive RGD modified titanium surfaces: a first step toward cell selective surfaces" 2008

13 Temple J, "Pin site care for preventing infections associated with external bone fixators and pins" 2004

14 Kikuchi Y, "Photocatalytic bactericidal effect of TiO2 thin films:dynamic view of the active oxygen species responsible for the effect" 1997

15 von Eiff C, "Pathogenesis of infections due to coagulase-negative staphylococci" 2002

16 Vincent JL, "Nosocomial infections in adult intensive-care units" 2003

17 Hope R, "Non-susceptibility trends among staphylococci from bacteraemias in the UK and Ireland, 2001-06" 2008

18 Gristina AG, "Implant failure and the immunoincompetent fibro-inflammatory zone" 1994

19 Elagli, "Evaluation de la tenue a la corrosion du titane en milieu salive artificielle" 1991

20 Brammer KS, "Enhanced cellular mobility guided by TiO2 nanotube surfaces" 2008

21 Lejeune P, "Contamination of abiotic surfaces: What a colonizing bacteria sees and how to blur it" 2003

22 Breme, "Comparision of titaniumtantalumand titanium-niobium alloys for application asdental implants" 1989

23 Rahme LG, "Common virulence factors for bacterial pathogenicity in plants and animals" 1995

24 Van Delden, "Cell-to-cell signaling and Pseudomonas aeruginosa infections" 1998

25 Elagli, "Biocompatibilite du Titane de ses alliages" 1989

26 Wei C, "Bactericidal activity of TiO2 photocatalyst in aquous media: toward a solar-assisted water disinfection system" 1994

27 Geesey GG, "Bacterial behaviour at surfaces" 2001

28 Dunne WM Jr, "Bacterial adhesion: Seen any good biofilms lately?" 2002

29 Kinnari TJ, "Bacterial adherence to titanium surface coated with human serum albumin" 2005

30 Suketa N, "An antibacterial surface on dental implants based on the photocatalytic bactericidal effect" 2005

31 Bergogne-Berezin E, "Acinetobacter spp As nosocomial pathogens: microbiological, clinical, and epidemiologicalfeatures" 1996

32 Peleg AY, "Acinetobacter baumannii: emergence of a successful pathogen" 2008

33 Sherertz RJ, "A three-year study of nosocomial infections associated with Pseudomonas aeruginosa" 1983