베나제프릴의 장관막 투과도와 흡수 클리어런스에 미치는 아목시실린의 영향 = Effect of Amoxicillin on the Intestinal Membrane Permeabiiity and Absorption Clearance of Benazepril

다국어 초록 (Multilingual Abstract)

Intestinal absorption of β-lactam antibiotics and angiotensin converting enzyme(ACE) inhibitors has been shown to use the carrier-mediated transport system. In vitro experiments have established that the efficacy of uptake by enterocytes depends on an inwardly directed proton gradient. It was suggested that benazepril was mediated by tripeptide transport system and that amoxicillin was transported by dipeptide transport carrier. The aim of this study is to assess the influence of amoxicillin on the intestinal absorption of benazepril using in vitro diffusion chamber and in situ single pass perfusion technique in the rat in order to elucidate whether the above transport systems are competitive or not. We obtained the gastrointestinal pemeability coefficient of amoxicillin, benazepril and both of them using in vitro diffusion chamber. And also the gastrointestinal absorption clearance of amoxicillin, benazepril and both of them using in situ single-pass perfusion method at steady state were calculated. Amoxicillin and benazepril were analyzed by HPLC. The results by the use of diffusion chamber in vitro indicated that the apparent intestinal permeability coefficient of benazepril was significantly(p$lt;0.01) decreased by amoxicillin(45.2%) and vice versa significantly(p$lt;0.01) decreased(89.1%). The results by the in situ gastrointestinal single-pass perfusion method indicated that the intestinal absorption clearance of benazepril was significantly(p$lt;0.05) decreased by amoxicillin (40.2%) and vice versa significantly(p$lt;0.05) decreased(54.8%). These results might suggest that they share the same peptide carrier pathway for oral absorption.